| IED ID | IndEnz0010001529 |
| Enzyme Type ID | esterase001529 |
| Protein Name |
Lysophospholipase NTE1 EC 3.1.1.5 Intracellular phospholipase B Neuropathy target esterase homolog |
| Gene Name | NTE1 CHGG_04763 |
| Organism | Chaetomium globosum (strain ATCC 6205 / CBS 148.51 / DSM 1962 / NBRC 6347 / NRRL 1970) (Soil fungus) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta sordariomyceta Sordariomycetes Sordariomycetidae Sordariales Chaetomiaceae Chaetomium Chaetomium globosum (Soil fungus) Chaetomium globosum (strain ATCC 6205 / CBS 148.51 / DSM 1962 / NBRC 6347 / NRRL 1970) (Soil fungus) |
| Enzyme Sequence | MDVVNSTARAAVTSATAVTAVTGTGDRHPNPLSSAVAAASDVANAHGSSSWLGLFARVVLWLLQFVSMVLYYAIKLATISVPTLLYTLFSTSLTVTMNATTLMLIVAAMIGAISWVVRYRYLNMYSRLPPEPQRKEPDVDLFPDTHEEGIKSGLSNYFDEFLSAIKIFGYLERPVFHELTRSMQTRKLIAGESFNLEEEKGFCLVVDGLVEIFVKSSSYNRRYPHGPYFSPNSEAPSSDDEHPAPGQQRYQLLTEVRNGAPMSSLFSIMSLFTEDVPLRHADEDNSEPGTTTHSGLFPNYPASADFRKSRVRMDSVPNTPQMDASASSSANNLPGQLDRGLPHVPPISLDGTGFSKPQRPVPKRSNTTSAHPDIIARATVDTTVAIIPASAFRRLIKIYPKATAHIVHVILSRFQRVTLATAYNYLGLTNEVLQIERQMLKYTTQQLPNHLRGDALDRLKEKFKREVERMGEEDDVSKGIALHNARAGRRRRSTATLRKEAALQAFSKQRNISSMSGSSLAIPNAGDLVTHLQQSRGGGNRAQSVAFTDGPSPHLDVEREAVSPLAQRTFDPFVTPRSIHVALEKRETLDEDNLFRESILECMFRSIGLTGSGGSNKDADSNQASPRLISFDQRRQKALYTNHAFGFMDGLDGSADGDTESITSAGLSLPASPNPQFLAQEMRDEMEIVFFPKGSVLVEQGERNPGLYYVVDGFLDICTQEDAAASDVVHPTSRTSLHAMDSAQSIRSPQRSPQPFAESMRSGNKVDDAEIKSKPNRRSVALIKPGGLAGYVGTISSYRSFIEVVAKTDVYVGFLPLTSIERIVDRYPIVLLTMAKRLTNLLPRLILHIDFALEWLQVNAGQVIFHEGDESEAIYIVLNGRLRLVEDRKDGGMNVKAEYGQGESIGELEVLTETSRSGTLHAIRDTELVKFPRTLFNSLAQEHPNITIKISKIIASRMRALIDDPSTMLGIKDSSGRSSINKSSTTLNLRTVAVLPVSAGVPVVEFSNRLLSALTEVGAPNGATSLNSAAVLNHLGKHAFNRMGKLKLSQYLADLEEKYGLVIYVADTNVNAPWTQTCVAQADCVLMVGLADGSPEIGEYERFMLGMKSTARKILVLLHQERYSNSGLTRKWLKNRVWINGGHFHVQMTYSPNAVPIHPPAKPSGPTLRERVQILQAEIQKYTSRKVRHSPFYSPDAPFKGDFHRLARRLCGKSVGLVLGGGGARGIAQIGIIRAMQEAGIPIDIVGGTSIGAFIGALYARHADFVPIVNAAKKFSGRMASMWRFALDLTYPSASYTTGHEFNRGIFKAFGNTQIEDFWLDYYCNTTNISKSRAEFHTSGYAWWYVRASMSLAGLLLPLCDEGSMLLDGGYVDNLTVSHMKSLGSDVIFAVDVGALDDNTPQAFGDSLSGMWAFFNRWNPFSSVANPPTLAEIQARLAYVSSVDALERAKTLPGCIYMRPPIEEYGTLDFGKFMEIYGVGYKYGQEFLAKLRERGGVLPIGEEMGGKKGLRRTMAPRRASI |
| Enzyme Length | 1521 |
| Uniprot Accession Number | Q2H0D3 |
| Absorption | |
| Active Site | ACT_SITE 1250; /note=Nucleophile; /evidence=ECO:0000255|PROSITE-ProRule:PRU01161; ACT_SITE 1368; /note=Proton acceptor; /evidence=ECO:0000255|PROSITE-ProRule:PRU01161 |
| Activity Regulation | ACTIVITY REGULATION: Inhibited by organophosphorus esters. {ECO:0000250}. |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=a 1-acyl-sn-glycero-3-phosphocholine + H2O = a fatty acid + H(+) + sn-glycerol 3-phosphocholine; Xref=Rhea:RHEA:15177, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:16870, ChEBI:CHEBI:28868, ChEBI:CHEBI:58168; EC=3.1.1.5; |
| DNA Binding | |
| EC Number | 3.1.1.5 |
| Enzyme Function | FUNCTION: Intracellular phospholipase B that catalyzes the double deacylation of phosphatidylcholine (PC) to glycerophosphocholine (GroPCho). Plays an important role in membrane lipid homeostasis. Responsible for the rapid PC turnover in response to inositol, elevated temperatures, or when choline is present in the growth medium (By similarity). {ECO:0000250}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | NP_BIND 670..789; /note=cNMP 1; NP_BIND 837..957; /note=cNMP 2 |
| Features | Active site (2); Chain (1); Compositional bias (2); Domain (1); Frameshift (1); Motif (3); Nucleotide binding (2); Region (3); Topological domain (3); Transmembrane (2) |
| Keywords | Endoplasmic reticulum;Hydrolase;Lipid degradation;Lipid metabolism;Membrane;Reference proteome;Repeat;Transmembrane;Transmembrane helix |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000250}; Multi-pass membrane protein {ECO:0000250}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | MOTIF 1221..1226; /note=GXGXXG; /evidence=ECO:0000255|PROSITE-ProRule:PRU01161; MOTIF 1248..1252; /note=GXSXG; /evidence=ECO:0000255|PROSITE-ProRule:PRU01161; MOTIF 1368..1370; /note=DGA/G; /evidence=ECO:0000255|PROSITE-ProRule:PRU01161 |
| Gene Encoded By | |
| Mass | 167,339 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:15177 |
| Cross Reference Brenda |