| IED ID | IndEnz0010001539 |
| Enzyme Type ID | esterase001539 |
| Protein Name |
Lysophospholipase NTE1 EC 3.1.1.5 Intracellular phospholipase B Neuropathy target esterase homolog |
| Gene Name | NTE1 CAALFM_CR08300CA CaO19.13754 CaO19.6396 |
| Organism | Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Saccharomycotina (true yeasts) Saccharomycetes Saccharomycetales Debaryomycetaceae Candida/Lodderomyces clade Candida Candida albicans (Yeast) Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast) |
| Enzyme Sequence | MGPEFEDSIPLVHSDNRTTTIYSVYIIISDIFSFVQWLLFKVLNLIIIDSPAFVLRLLSKNFEINLHLSSILATLIGVSVVTYLVIRYKFLTGYSHSNDQKEGAGKKGIHPASSKVFVGKSKQDKKPSNYLDEFLLAIKVFGYLDKAVFHELTKSMTTQKLSHEEILCLDESLGFSIVVEGVAQVYTKKSKENSSKYKQRYFEEIEEERDEFLILGDKQYQLLNEVKSGAPLSSLFSTLDLFRPRNKNEEMALTPNDEDGNSVVALNMDYLSKSEPATDSKLSNSDDDENGQDYPEIIVRPKKSHNNGTITIAIIPHTAFERVQSKYPKATSHIVTMVLTRLYKVTMSTVQNYLGLTSEILKSEIKLNEENATSLPRYFVDGLLERLNKKEEAEAEAENLPKKLKHHHRNQLQRTTSRYVSLDSKVKSNHPGDLLSSVPISRSEFQKKVLPKSSTTSLSDGDNKRMNFTDEMEETEENSLRVAIIENIFKMVGIEETNGIVERTGYFQSLSSSTSSSIVGLDSLNQSIMSGATTPTTKRSHPVFNNQGSLMNTINLAELRKPSTAENSTLPKLSGKKRLLSDMNIKDAFAKSLEIKYIGPDSTIVSQNSAITGLYYVIDGSLEIYNRSADVSAPNRYIYTVESGGIAGYLTSVVGFRSMVTIKTPKKTGAVVAYIPKNDYNKLLDKYYFLQLPVALKLKNLLSKQILTIDYALEWCHIPAGEVLCSQGDLANGFHVVLSGRFRVVRSTNKNTEKEDVEVLGEYGHGESIGEVEVLTASRRSNTLIAVRDSETARIPRSLFEMLSNENPSIMVSVSRLVASKVLASQYQPRERNFITSTTSQESFTSANYKTITILPTVSGLPVRQFAEKLVQALRQIGRNVIALDQASILTHLGRHAFDESLARLKLSGYFAYLEEEYETIVYICDTPVQSNWTSTCISQGDCILLLADAEDDSTTIGEYEQLLVKLKTTARTDLCLLHQDKVVRAGSTSPWLKNRIWVQGHHHIQMKFEQGANVLPHKKSFISDLAAKLSQNKAIKSTFEATKQHIKWYVKENDQSKVLKIYKDDFMRLARILSNEAVGLVLGGGGSRGISHVGVVTSLERHGIPVDIIGGTSIGSFVGGLYAKEYNIVSIYAMAKKFSKRISSVWRMLFDLTYPVTSYITGYEFNRGIWKVFGFAEIEDFWIKYFCNSANITNSTMDIHEKGYAWRFIRASMSLAGLLPPIAFNGCMLLDGGYLDNLPVNEMKKRGVKHIIAVDVGSVDDRTPMNYGDTLSGFWVLLNRWNPFSSHPNIPTMMDIQMRLTYVSSVNALEEAKRTPGVYYLRPPIDPYATLDFGKFDEIYKVGLKYADDLFADWKSKGKFPRIAGLVEKKKDGEEKKILYRRNSI |
| Enzyme Length | 1386 |
| Uniprot Accession Number | Q5A368 |
| Absorption | |
| Active Site | ACT_SITE 1114; /note=Nucleophile; /evidence=ECO:0000255|PROSITE-ProRule:PRU01161; ACT_SITE 1232; /note=Proton acceptor; /evidence=ECO:0000255|PROSITE-ProRule:PRU01161 |
| Activity Regulation | ACTIVITY REGULATION: Inhibited by organophosphorus esters. {ECO:0000250}. |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=a 1-acyl-sn-glycero-3-phosphocholine + H2O = a fatty acid + H(+) + sn-glycerol 3-phosphocholine; Xref=Rhea:RHEA:15177, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:16870, ChEBI:CHEBI:28868, ChEBI:CHEBI:58168; EC=3.1.1.5; |
| DNA Binding | |
| EC Number | 3.1.1.5 |
| Enzyme Function | FUNCTION: Intracellular phospholipase B that catalyzes the double deacylation of phosphatidylcholine (PC) to glycerophosphocholine (GroPCho). Plays an important role in membrane lipid homeostasis. Responsible for the rapid PC turnover in response to inositol, elevated temperatures, or when choline is present in the growth medium (By similarity). {ECO:0000250}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | NP_BIND 577..701; /note=cNMP 1; NP_BIND 697..821; /note=cNMP 2 |
| Features | Active site (2); Chain (1); Domain (1); Motif (3); Nucleotide binding (2); Region (1); Topological domain (3); Transmembrane (2) |
| Keywords | Endoplasmic reticulum;Hydrolase;Lipid degradation;Lipid metabolism;Membrane;Reference proteome;Repeat;Transmembrane;Transmembrane helix |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000250}; Multi-pass membrane protein {ECO:0000250}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | MOTIF 1085..1090; /note=GXGXXG; /evidence=ECO:0000255|PROSITE-ProRule:PRU01161; MOTIF 1112..1116; /note=GXSXG; /evidence=ECO:0000255|PROSITE-ProRule:PRU01161; MOTIF 1232..1234; /note=DGA/G; /evidence=ECO:0000255|PROSITE-ProRule:PRU01161 |
| Gene Encoded By | |
| Mass | 155,704 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:15177 |
| Cross Reference Brenda |