| IED ID | IndEnz0010001572 |
| Enzyme Type ID | esterase001572 |
| Protein Name |
Acyl-coenzyme A thioesterase 11 Acyl-CoA thioesterase 11 EC 3.1.2.- Acyl-CoA thioester hydrolase 11 Adipose-associated thioesterase Brown fat-inducible thioesterase BFIT Palmitoyl-coenzyme A thioesterase EC 3.1.2.2 |
| Gene Name | Acot11 Bfit Thea |
| Organism | Mus musculus (Mouse) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Euarchontoglires Glires (Rodents and rabbits) Rodentia Myomorpha (mice and others) Muroidea Muridae Murinae Mus Mus Mus musculus (Mouse) |
| Enzyme Sequence | MIQNVGNHLRRGFASMFSNRTSRKSISHPESGDPPTMAEGEGYRNPTEVQMSQLVLPCHTNHRGELSIGQLLKWIDTTACLSAERHAGCPCVTASMDDIYFDHTISVGQVVNIKAKVNRAFNSSMEVGIQVVSEDLCSEKQWSVCKALATFVAHRELSKVKLKQVIPLTEEEKTEHGVAAERRRMRLVYADTIKDLLTHCVIQDDLDKDCSNMVPAEKTRVESVELVLPPHANHQGNTFGGQIMAWMENVATIAASRLCHAHPTLKAIEMFHFRGPSQVGDRLVLKAIVNNAFKHSMEVGVCVEAYRQEAETQRRHINSAFMTFVVLDKDDQPQKLPWIRPQPGEGERRYREASARKKIRLDRKYLVSCKQAEVALSVPWDPSNQVYLSYYNVSSLKTLMAKDNWVLSVEISEVRLYILEEDFLSFHLEMVVNVDAAQVFQLLSDLRRRPEWDKHYRSVELVQQVDEDDAIYHVISPALSGNTKPQDFVILASRRKPCDNGDPYVIALRSVTLPTHHETPEYQRGETLCSGFCLWREGDQMTKVSYYNQATPGFLNYVTTNVSGLSSEFYNTFKACESFLLDNRNDLAPSLQTL |
| Enzyme Length | 594 |
| Uniprot Accession Number | Q8VHQ9 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | BINDING 183; /note=Coenzyme A; /evidence=ECO:0000250 |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=H2O + hexadecanoyl-CoA = CoA + H(+) + hexadecanoate; Xref=Rhea:RHEA:16645, ChEBI:CHEBI:7896, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:57287, ChEBI:CHEBI:57379; EC=3.1.2.2; Evidence={ECO:0000250|UniProtKB:Q8WXI4};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:16646; Evidence={ECO:0000250|UniProtKB:Q8WXI4}; CATALYTIC ACTIVITY: Reaction=H2O + tetradecanoyl-CoA = CoA + H(+) + tetradecanoate; Xref=Rhea:RHEA:40119, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30807, ChEBI:CHEBI:57287, ChEBI:CHEBI:57385; Evidence={ECO:0000250|UniProtKB:Q8WXI4};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:40120; Evidence={ECO:0000250|UniProtKB:Q8WXI4}; CATALYTIC ACTIVITY: Reaction=dodecanoyl-CoA + H2O = CoA + dodecanoate + H(+); Xref=Rhea:RHEA:30135, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:18262, ChEBI:CHEBI:57287, ChEBI:CHEBI:57375; Evidence={ECO:0000250|UniProtKB:Q8WXI4};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:30136; Evidence={ECO:0000250|UniProtKB:Q8WXI4}; CATALYTIC ACTIVITY: Reaction=butanoyl-CoA + H2O = butanoate + CoA + H(+); Xref=Rhea:RHEA:40111, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17968, ChEBI:CHEBI:57287, ChEBI:CHEBI:57371; Evidence={ECO:0000250|UniProtKB:Q8WXI4};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:40112; Evidence={ECO:0000250|UniProtKB:Q8WXI4}; |
| DNA Binding | |
| EC Number | 3.1.2.-; 3.1.2.2 |
| Enzyme Function | FUNCTION: Has an acyl-CoA thioesterase activity with a preference for the long chain fatty acyl-CoA thioesters hexadecanoyl-CoA/palmitoyl-CoA and tetradecanoyl-CoA/myristoyl-CoA which are the main substrates in the mitochondrial beta-oxidation pathway. {ECO:0000250|UniProtKB:Q8WXI4}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | PATHWAY: Lipid metabolism; fatty acid metabolism. {ECO:0000250|UniProtKB:Q8WXI4}. |
| nucleotide Binding | |
| Features | Binding site (1); Chain (1); Domain (3); Modified residue (2); Region (4); Transit peptide (1) |
| Keywords | Cytoplasm;Fatty acid metabolism;Hydrolase;Lipid metabolism;Mitochondrion;Phosphoprotein;Reference proteome;Repeat;Serine esterase;Transit peptide |
| Interact With | |
| Induction | INDUCTION: By cold exposure and repressed by heat exposure. |
| Subcellular Location | SUBCELLULAR LOCATION: Mitochondrion matrix {ECO:0000250|UniProtKB:Q8WXI4}. Cytoplasm {ECO:0000250|UniProtKB:Q8WXI4}. |
| Modified Residue | MOD_RES 15; /note=Phosphoserine; /evidence=ECO:0007744|PubMed:21183079; MOD_RES 25; /note=Phosphoserine; /evidence=ECO:0007744|PubMed:21183079 |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 11217851; 12466851; 12693553; 16103133; 16141072; 21677750; 22427358; 22993230; 27110486; 29208699; 29688375; 34108467; |
| Motif | |
| Gene Encoded By | |
| Mass | 67,355 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:16645; RHEA:16646; RHEA:40119; RHEA:40120; RHEA:30135; RHEA:30136; RHEA:40111; RHEA:40112 |
| Cross Reference Brenda | 3.1.2.20; |