Detail Information for IndEnz0010001577
IED ID IndEnz0010001577
Enzyme Type ID esterase001577
Protein Name Pimeloyl-
acyl-carrier protein methyl ester esterase
EC 3.1.1.85
Biotin synthesis protein BioH
Carboxylesterase BioH
Gene Name bioH AZOSEA34330 ebA6017
Organism Aromatoleum aromaticum (strain EbN1) (Azoarcus sp. (strain EbN1))
Taxonomic Lineage cellular organisms Bacteria Proteobacteria Betaproteobacteria Rhodocyclales Rhodocyclaceae Aromatoleum Aromatoleum aromaticum Aromatoleum aromaticum (strain EbN1) (Azoarcus sp. (strain EbN1))
Enzyme Sequence MNRPDLVLLHGWGLGPQVWSALTPYLPAGLRVRTPALPGHGGTPARGPTLEAWSDALLPELPDDAVVCGWSLGGLVALDLARRHPHKVARLVLIGTSPCFVTRPENAAAPWPYGLAASTVTGFIDDFAHDPAATLRRFVALQALGDARRRTVSNALNAALANLEQCRPAALGAGLELLADTDWRAALDDVRQPVQLIHGAGDALMPLAAAEWLATRLPDARLARFDDCGHAPFLSHPEDCAVLIEDVVRG
Enzyme Length 250
Uniprot Accession Number Q5NZF6
Absorption
Active Site ACT_SITE 71; /note=Nucleophile; /evidence=ECO:0000255|HAMAP-Rule:MF_01260; ACT_SITE 202; /evidence=ECO:0000255|HAMAP-Rule:MF_01260; ACT_SITE 230; /evidence=ECO:0000255|HAMAP-Rule:MF_01260
Activity Regulation
Binding Site BINDING 12; /note=Substrate; via amide nitrogen and carbonyl oxygen; /evidence=ECO:0000255|HAMAP-Rule:MF_01260; BINDING 230; /note=Substrate; /evidence=ECO:0000255|HAMAP-Rule:MF_01260
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=6-carboxyhexanoyl-[ACP] methyl ester + H2O = 6-carboxyhexanoyl-[ACP] + H(+) + methanol; Xref=Rhea:RHEA:42700, Rhea:RHEA-COMP:9955, Rhea:RHEA-COMP:10186, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17790, ChEBI:CHEBI:78846, ChEBI:CHEBI:82735; EC=3.1.1.85; Evidence={ECO:0000255|HAMAP-Rule:MF_01260};
DNA Binding
EC Number 3.1.1.85
Enzyme Function FUNCTION: The physiological role of BioH is to remove the methyl group introduced by BioC when the pimeloyl moiety is complete. It allows to synthesize pimeloyl-ACP via the fatty acid synthetic pathway through the hydrolysis of the ester bonds of pimeloyl-ACP esters. {ECO:0000255|HAMAP-Rule:MF_01260}.
Temperature Dependency
PH Dependency
Pathway PATHWAY: Cofactor biosynthesis; biotin biosynthesis. {ECO:0000255|HAMAP-Rule:MF_01260}.
nucleotide Binding
Features Active site (3); Binding site (2); Chain (1); Region (2)
Keywords Biotin biosynthesis;Cytoplasm;Hydrolase;Reference proteome;Serine esterase
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01260}.
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 26,598
Kinetics
Metal Binding
Rhea ID RHEA:42700
Cross Reference Brenda