| IED ID | IndEnz0010001596 |
| Enzyme Type ID | esterase001596 |
| Protein Name |
Acetylesterase EC 3.1.1.6 Carbohydrate esterase family 16 protein |
| Gene Name | aes1 TrAE |
| Organism | Hypocrea jecorina (Trichoderma reesei) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta sordariomyceta Sordariomycetes Hypocreomycetidae Hypocreales Hypocreaceae Trichoderma Hypocrea jecorina (Trichoderma reesei) |
| Enzyme Sequence | MRSILVIPSFVAVLNAFSLFPKPHDDFKYLITFGDSYTDNGRLGYYGSHQAHGPPPGVMPPEANVTASGGLQWPQYVEASTGATLYDYAIAGATCDNNNVERWAAFMNANYPSIITDEIPSFKADRKTKLYRGVTSANTVYALWIGTNDLSYTGILSDSQVKGTNITTYIDCLWNVFDAIHAAGGRRFVILNNNALQLTGLYRPLSDGGAGDNQFWQNKTLYNQTEYAQKMLEYTTSSNTMIDYGVPFHLLVKNRWPGSKVAVYDIHSLIMDIYNQPSRYLEPPHNVVGYYKHCDVNGTNCLYGPGRLDSYLWYDELHPSNIIASYIAREFLNVVSGRSKYGTYWEHW |
| Enzyme Length | 348 |
| Uniprot Accession Number | A7J2C6 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=an acetyl ester + H2O = acetate + an aliphatic alcohol + H(+); Xref=Rhea:RHEA:12957, ChEBI:CHEBI:2571, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30089, ChEBI:CHEBI:47622; EC=3.1.1.6; Evidence={ECO:0000269|PubMed:18978092, ECO:0000269|PubMed:24140638, ECO:0000269|Ref.2}; |
| DNA Binding | |
| EC Number | 3.1.1.6 |
| Enzyme Function | FUNCTION: Acetylesterase that acts as an exo-deacetylase (PubMed:24140638). Shows activity towards naphtyl acetate, triacetin, as well as towards glucose- and xylose acetates (PubMed:18978092, Ref.2). Liberates acetic acid from xylo-oligomers (PubMed:18978092, Ref.2, PubMed:24140638). {ECO:0000269|PubMed:18978092, ECO:0000269|PubMed:24140638, ECO:0000269|Ref.2}. |
| Temperature Dependency | BIOPHYSICOCHEMICAL PROPERTIES: Temperature dependence: Optimum temperature is 50 degrees Celsius. {ECO:0000269|Ref.2}; |
| PH Dependency | BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 5.5. {ECO:0000269|Ref.2}; |
| Pathway | |
| nucleotide Binding | |
| Features | Chain (1); Glycosylation (5); Signal peptide (1) |
| Keywords | Glycoprotein;Hydrolase;Secreted;Signal |
| Interact With | |
| Induction | INDUCTION: Expression is induced by sophorose, cellulose, oat spelt xylan, lactose, and arabinose. {ECO:0000269|PubMed:18978092}. |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000305}. |
| Modified Residue | |
| Post Translational Modification | PTM: N-glycosylated. {ECO:0000269|PubMed:18978092}. |
| Signal Peptide | SIGNAL 1..16; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 39,158 |
| Kinetics | BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=0.23 mM for 4-nitrophenyl acetate {ECO:0000269|PubMed:18978092}; Vmax=20.5 umol/min/mg enzyme toward 4-nitrophenyl acetate {ECO:0000269|PubMed:18978092}; |
| Metal Binding | |
| Rhea ID | RHEA:12957 |
| Cross Reference Brenda | 3.1.1.6; |