| IED ID | IndEnz0010001608 |
| Enzyme Type ID | esterase001608 |
| Protein Name |
Carboxymethylenebutenolidase EC 3.1.1.45 Dienelactone hydrolase DLH |
| Gene Name | clcD |
| Organism | Pseudomonas knackmussii (strain DSM 6978 / LMG 23759 / B13) |
| Taxonomic Lineage | cellular organisms Bacteria Proteobacteria Gammaproteobacteria Pseudomonadales Pseudomonadaceae Pseudomonas Pseudomonas knackmussii Pseudomonas knackmussii (strain DSM 6978 / LMG 23759 / B13) |
| Enzyme Sequence | MLTEGISIQSYDGHTFGALVGSPAKAPAPVIVIAQEIFGVNAFMRETVSWLVDQGYAAVCPDLYARQAPGTALDPQDERQREQAYKLWQAFDMEAGVGDLEAAIRYARHQPYSNGKVGLVGYCLGGALAFLVAAKGYVDRAVGYYGVGLEKQLKKVPEVKHPALFHMGGQDHFVPAPSRQLITEGFGANPLLQVHWYEEAGHSFARTSSSGYVASAAALANERRLDFLAPLQSKKP |
| Enzyme Length | 236 |
| Uniprot Accession Number | P0A115 |
| Absorption | |
| Active Site | ACT_SITE 123; ACT_SITE 171; ACT_SITE 202 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=2-(5-oxo-2,5-dihydrofuran-2-ylidene)acetate + H2O = 4-oxohex-2-enedioate + H(+); Xref=Rhea:RHEA:12372, ChEBI:CHEBI:12040, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:57263; EC=3.1.1.45; |
| DNA Binding | |
| EC Number | 3.1.1.45 |
| Enzyme Function | FUNCTION: Ring cleavage of cyclic ester dienelactone to produce maleylacetate. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | PATHWAY: Aromatic compound metabolism; 3-chlorocatechol degradation. |
| nucleotide Binding | |
| Features | Active site (3); Beta strand (7); Chain (1); Helix (10); Mutagenesis (1); Turn (1) |
| Keywords | 3D-structure;Aromatic hydrocarbons catabolism;Hydrolase;Plasmid;Serine esterase |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | X-ray crystallography (8) |
| Cross Reference PDB | 1DIN; 4P93; 4U2B; 4U2C; 4U2D; 4U2E; 4U2F; 4U2G; |
| Mapped Pubmed ID | 25005082; 25419863; |
| Motif | |
| Gene Encoded By | Plasmid pB13 |
| Mass | 25,555 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:12372 |
| Cross Reference Brenda | 3.1.1.45; |