Detail Information for IndEnz0010001609
IED ID IndEnz0010001609
Enzyme Type ID esterase001609
Protein Name Carboxymethylenebutenolidase
EC 3.1.1.45
Dienelactone hydrolase
DLH
Gene Name clcD
Organism Pseudomonas putida (Arthrobacter siderocapsulatus)
Taxonomic Lineage cellular organisms Bacteria Proteobacteria Gammaproteobacteria Pseudomonadales Pseudomonadaceae Pseudomonas Pseudomonas putida group Pseudomonas putida (Arthrobacter siderocapsulatus)
Enzyme Sequence MLTEGISIQSYDGHTFGALVGSPAKAPAPVIVIAQEIFGVNAFMRETVSWLVDQGYAAVCPDLYARQAPGTALDPQDERQREQAYKLWQAFDMEAGVGDLEAAIRYARHQPYSNGKVGLVGYCLGGALAFLVAAKGYVDRAVGYYGVGLEKQLKKVPEVKHPALFHMGGQDHFVPAPSRQLITEGFGANPLLQVHWYEEAGHSFARTSSSGYVASAAALANERRLDFLAPLQSKKP
Enzyme Length 236
Uniprot Accession Number P0A114
Absorption
Active Site ACT_SITE 123; ACT_SITE 171; ACT_SITE 202
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=2-(5-oxo-2,5-dihydrofuran-2-ylidene)acetate + H2O = 4-oxohex-2-enedioate + H(+); Xref=Rhea:RHEA:12372, ChEBI:CHEBI:12040, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:57263; EC=3.1.1.45;
DNA Binding
EC Number 3.1.1.45
Enzyme Function FUNCTION: Ring cleavage of cyclic ester dienelactone to produce maleylacetate.
Temperature Dependency
PH Dependency
Pathway PATHWAY: Aromatic compound metabolism; 3-chlorocatechol degradation.
nucleotide Binding
Features Active site (3); Beta strand (8); Chain (1); Helix (10); Turn (1)
Keywords 3D-structure;Aromatic hydrocarbons catabolism;Hydrolase;Plasmid;Serine esterase
Interact With
Induction
Subcellular Location
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D X-ray crystallography (10)
Cross Reference PDB 1GGV; 1ZI6; 1ZI8; 1ZI9; 1ZIC; 1ZIX; 1ZIY; 1ZJ4; 1ZJ5; 4P92;
Mapped Pubmed ID 11053834; 15983415; 25005082;
Motif
Gene Encoded By Plasmid pAC27
Mass 25,555
Kinetics
Metal Binding
Rhea ID RHEA:12372
Cross Reference Brenda