| IED ID | IndEnz0010001629 |
| Enzyme Type ID | esterase001629 |
| Protein Name |
Streptogrisin-B EC 3.4.21.81 Pronase enzyme B SGPB Serine protease B |
| Gene Name | sprB |
| Organism | Streptomyces griseus |
| Taxonomic Lineage | cellular organisms Bacteria Terrabacteria group Actinobacteria Actinomycetia (high G+C Gram-positive bacteria) Streptomycetales Streptomycetaceae Streptomyces Streptomyces griseus group Streptomyces griseus subgroup Streptomyces griseus |
| Enzyme Sequence | MRIKRTSNRSNAARRVRTTAVLAGLAAVAALAVPTANAETPRTFSANQLTAASDAVLGADIAGTAWNIDPQSKRLVVTVDSTVSKAEINQIKKSAGANADALRIERTPGKFTKLISGGDAIYSSTGRCSLGFNVRSGSTYYFLTAGHCTDGATTWWANSARTTVLGTTSGSSFPNNDYGIVRYTNTTIPKDGTVGGQDITSAANATVGMAVTRRGSTTGTHSGSVTALNATVNYGGGDVVYGMIRTNVCAEPGDSGGPLYSGTRAIGLTSGGSGNCSSGGTTFFQPVTEALSAYGVSVY |
| Enzyme Length | 299 |
| Uniprot Accession Number | P00777 |
| Absorption | |
| Active Site | ACT_SITE 147; /note=Charge relay system; ACT_SITE 177; /note=Charge relay system; ACT_SITE 255; /note=Charge relay system |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Hydrolysis of proteins with trypsin-like specificity.; EC=3.4.21.81; |
| DNA Binding | |
| EC Number | 3.4.21.81 |
| Enzyme Function | FUNCTION: Has a primary specificity for large aliphatic or aromatic amino acids. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Beta strand (17); Chain (1); Disulfide bond (2); Helix (2); Propeptide (1); Sequence conflict (4); Signal peptide (1); Turn (2) |
| Keywords | 3D-structure;Direct protein sequencing;Disulfide bond;Hydrolase;Protease;Serine protease;Signal;Zymogen |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..38 |
| Structure 3D | X-ray crystallography (28) |
| Cross Reference PDB | 1CSO; 1CT0; 1CT2; 1CT4; 1DS2; 1SGD; 1SGE; 1SGN; 1SGP; 1SGQ; 1SGR; 1SGY; 2GKV; 2NU0; 2NU1; 2NU2; 2NU3; 2NU4; 2QA9; 2QAA; 2SGD; 2SGE; 2SGF; 2SGP; 2SGQ; 3SGB; 3SGQ; 4SGB; |
| Mapped Pubmed ID | 10739250; 11162096; 17266986; 18157955; 6414511; 8535235; |
| Motif | |
| Gene Encoded By | |
| Mass | 30,554 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda | 3.4.21.81; |