| IED ID | IndEnz0010001630 |
| Enzyme Type ID | esterase001630 |
| Protein Name |
Neuropathy target esterase sws Swiss cheese DSWS EC 3.1.1.5 |
| Gene Name | sws CG2212 |
| Organism | Drosophila melanogaster (Fruit fly) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Protostomia Ecdysozoa Panarthropoda Arthropoda Mandibulata Pancrustacea Hexapoda Insecta Dicondylia Pterygota (winged insects) Neoptera Endopterygota Diptera Brachycera Muscomorpha Eremoneura Cyclorrhapha Schizophora Acalyptratae Ephydroidea Drosophilidae (pomace flies) Drosophilinae Drosophilini Drosophila (fruit flies) Sophophora melanogaster group melanogaster subgroup Drosophila melanogaster (Fruit fly) |
| Enzyme Sequence | MDVLEMLRASASGSYNTIFSDAWCQYVSKQITATVYMYFALVMMSLLFIAWFLYFKRMARLRLRDEIARSISTVTNSSGDMRGLRFRKRDKMLFYGRRMLRKMKNVSGQMYSSGKGYKRRAVMRFARRILQLRRDNMPLEMRTVEPPAEYLEETIEGSDRVPPDALYMLQSIRIFGHFEKPVFLRLCKHTQLLELMAGDYLFKITDPDDSVYIVQSGMINVYISNADGSTLSLKTVRKGESVTSLLSFIDVLSGNPSYYKTVTAKAIEKSVVIRLPMQAFEEVFQDNPDVMIRVIQVIMIRLQRVLFTALRNYLGLNAELVQNHMRYKSVSTMSGPINSQTSQSSRQAPNGPPMVISQMNLMQSAVSGTGSSGVSVTVTRPPSSPSRHSREEHTLSDPNPNPDGSFHGTTNLFTEVHGDAPNADLFHQQQQQHSVGNLSTRRSSITLMAPDGSHSCLQTPGVTTSIDMRLVQSSAVDSLRKELGLSEEDSHIIEPFVELRELEPNVTLITEGNADDVCVWFVMTGTLAVYQSNQDATRAKQDKSDMLIHFVHPGEIVGGLAMLTGEASAYTIRSRSITRIAFIRRAAIYQIMRQRPRIVLDLGNGVVRRLSPLVRQCDYALDWIFLESGRAVYRQDESSDSTYIVLSGRMRSVITHPGGKKEIVGEYGKGDLVGIVEMITETSRTTTVMAVRDSELAKLPEGLFNAIKLRYPIVVTKLISFLSHRFLGSMQTRSGSGAPGAPVEANPVTHKYSTVALVPITDEVPMTPFTYELYHSLCAIGPVLRLTSDVVRKQLGSNIFEAANEYRLTSWLAQQEDRNIITLYQCDSSLSAWTQRCMRQADVILIVGLGDRSHLVGKFEREIDRLAMRTQKELVLLYPEASNAKPANTLSWLNARPWVTKHHHVLCVKRIFTRKSQYRINDLYSRVLLSEPNMHSDFSRLARWLTGNSIGLVLGGGGARGAAHIGMLKAIQEAGIPVDMVGGVSIGALMGALWCSERNITTVTQKAREWSKKMTKWFLQLLDLTYPITSMFSGREFNKTIHDTFGDVSIEDLWIPYFTLTTDITASCHRIHTNGSLWRYVRSSMSLSGYMPPLCDPKDGHLLLDGGYVNNLPADVMHNLGAAHIIAIDVGSQDDTDLTNYGDDLSGWWLLYKKWNPFTSPVKVPDLPDIQSRLAYVSCVRQLEEVKNSDYCEYIRPPIDKYKTLAFGSFDEIRDVGYVFGKNYFESMAKAGRLGRFNQWFNKEPPKRVNHASLNEYTFIDLAQIVCRLPETYAVNTAELFSEDEDCDGYISEPTTLNTDRRRIQVSRAGNSLSFSETEMDSDVELDLKLERKTDKSTQSSPPSNSRSDMRGKEEARHMSNWHWGVKHKDETGSGATEATKTQTGQEQELQQEQQDQGATAEQLVDKDKEENKENRSSPNNETKN |
| Enzyme Length | 1425 |
| Uniprot Accession Number | Q9U969 |
| Absorption | |
| Active Site | ACT_SITE 985; /note=Nucleophile; /evidence=ECO:0000255|PROSITE-ProRule:PRU01161; ACT_SITE 1105; /note=Proton acceptor; /evidence=ECO:0000255|PROSITE-ProRule:PRU01161 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=a 1-acyl-sn-glycero-3-phosphocholine + H2O = a fatty acid + H(+) + sn-glycerol 3-phosphocholine; Xref=Rhea:RHEA:15177, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:16870, ChEBI:CHEBI:28868, ChEBI:CHEBI:58168; EC=3.1.1.5; Evidence={ECO:0000269|PubMed:15772346}; |
| DNA Binding | |
| EC Number | 3.1.1.5 |
| Enzyme Function | FUNCTION: Phospholipase B that deacylates intracellular phosphatidylcholine (PtdCho), generating glycerophosphocholine (GroPtdCho). This deacylation occurs at both sn-2 and sn-1 positions of PtdCho. Its specific chemical modification by certain organophosphorus (OP) compounds leads to distal axonopathy. Plays a role in the signaling mechanism between neurons and glia that regulates glia wrapping during development of the adult brain. Essential for membrane lipid homeostasis and cell survival in both neurons and glia of the adult brain. {ECO:0000269|PubMed:15772346, ECO:0000269|PubMed:18945896, ECO:0000269|PubMed:9295388}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | NP_BIND 174..301; /note=cNMP 1; NP_BIND 482..609; /note=cNMP 2; NP_BIND 598..727; /note=cNMP 3 |
| Features | Active site (2); Alternative sequence (3); Chain (1); Compositional bias (4); Domain (1); Erroneous initiation (1); Modified residue (3); Motif (3); Mutagenesis (4); Nucleotide binding (3); Region (2); Sequence conflict (7); Topological domain (2); Transmembrane (1) |
| Keywords | Alternative splicing;Developmental protein;Endoplasmic reticulum;Hydrolase;Lipid degradation;Lipid metabolism;Membrane;Neurogenesis;Phosphoprotein;Reference proteome;Transmembrane;Transmembrane helix |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000269|PubMed:15772346, ECO:0000269|PubMed:18945896}; Single-pass type I membrane protein {ECO:0000269|PubMed:15772346, ECO:0000269|PubMed:18945896}. Note=Sws tethers Pka-C3 to the membrane. |
| Modified Residue | MOD_RES 444; /note=Phosphoserine; /evidence=ECO:0000269|PubMed:18327897; MOD_RES 453; /note=Phosphoserine; /evidence=ECO:0000269|PubMed:18327897; MOD_RES 1160; /note=Phosphoserine; /evidence=ECO:0000269|PubMed:18327897 |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 10729831; 10816586; 11948694; 12610523; 12618402; 12696671; 14960014; 17194782; 1723709; 19761846; 19781664; 20074523; 20220848; 20371351; 21074052; 2121923; 2123349; 21447707; 22227001; 22266106; 22583150; 22974305; 23071443; 24025855; 24508822; 24558370; 25033069; 25242144; 25294943; 25312911; 25511196; 25574898; 25687947; 25736969; 26000238; 26413411; 26551273; 26634819; 26671664; 26859824; 27794539; 28060320; 30027908; 30664368; 31068592; 31233884; 31722958; 31780887; 32096904; 32518884; 32660023; 32989326; 33801404; 34361042; 34506510; 34713801; 35055857; 8163553; 8656284; 8664555; 8794094; 8984732; 9811597; |
| Motif | MOTIF 956..961; /note=GXGXXG; /evidence=ECO:0000255|PROSITE-ProRule:PRU01161; MOTIF 983..987; /note=GXSXG; /evidence=ECO:0000255|PROSITE-ProRule:PRU01161; MOTIF 1105..1107; /note=DGA/G; /evidence=ECO:0000255|PROSITE-ProRule:PRU01161 |
| Gene Encoded By | |
| Mass | 160,573 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:15177 |
| Cross Reference Brenda |