| IED ID | IndEnz0010001641 |
| Enzyme Type ID | esterase001641 |
| Protein Name |
Poly 3-hydroxybutyrate depolymerase PHB depolymerase EC 3.1.1.75 |
| Gene Name | |
| Organism | Ralstonia pickettii (Burkholderia pickettii) |
| Taxonomic Lineage | cellular organisms Bacteria Proteobacteria Betaproteobacteria Burkholderiales Burkholderiaceae Ralstonia Ralstonia pickettii (Burkholderia pickettii) |
| Enzyme Sequence | MVRRLWRRIAGWLAACVAILCAFPLHAATAGPGAWSSQQTWAADSVNGGNLTGYFYWPASQPTTPNGKRALVLVLHGCVQTASGDVIDNANGAGFNWKSVADQYGAVILAPNATGNVYSNHCWDYANASPSRTAGHVGVLLDLVNRFVTNSQYAIDPNQVYVAGLSSGGGMTMVLGCIAPDIFAGIGINAGPPPGTTTAQIGYVPSGFTATTAANKCNAWAGSNAGKFSTQIAGAVWGTSDYTVAQAYGPMDAAAMRLVYGGNFTQGSQVSISGGGTNTPYTDSNGKVRTHEISVSGMAHAWPAGTGGDNTNYVDATHINYPVFVMDYWVKNNLRAGSGTGQAGSAPTGLAVTATTSTSVSLSWNAVANASSYGVYRNGSKVGSATATAYTDSGLIAGTTYSYTVTAVDPTAGESQPSAAVSATTKSAFTCTATTASNYAHVQAGRAHDSGGIAYANGSNQSMGLDNLFYTSTLAQTAAGYYIVGNCP |
| Enzyme Length | 488 |
| Uniprot Accession Number | P12625 |
| Absorption | |
| Active Site | ACT_SITE 166; /note=Charge relay system; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=[(3R)-hydroxybutanoate](n) + H2O = (3R)-hydroxybutanoate dimer + [(3R)-hydroxybutanoate](n-2) + H(+); Xref=Rhea:RHEA:56392, Rhea:RHEA-COMP:14464, Rhea:RHEA-COMP:14519, ChEBI:CHEBI:8298, ChEBI:CHEBI:10979, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378; EC=3.1.1.75; CATALYTIC ACTIVITY: Reaction=[(3R)-hydroxybutanoate](n) + H2O = (3R)-hydroxybutanoate trimer + [(3R)-hydroxybutanoate](n-3) + H(+); Xref=Rhea:RHEA:56396, Rhea:RHEA-COMP:14464, Rhea:RHEA-COMP:14520, ChEBI:CHEBI:8298, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:140385; EC=3.1.1.75; CATALYTIC ACTIVITY: Reaction=[(3R)-hydroxybutanoate](n) + H2O = (R)-3-hydroxybutanoate + [(3R)-hydroxybutanoate](n-1) + H(+); Xref=Rhea:RHEA:11248, Rhea:RHEA-COMP:14464, Rhea:RHEA-COMP:14518, ChEBI:CHEBI:8298, ChEBI:CHEBI:10983, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378; EC=3.1.1.75; CATALYTIC ACTIVITY: Reaction=[(3R)-hydroxybutanoate](n) + H2O = (3R)-hydroxybutanoate pentamer + [(3R)-hydroxybutanoate](n-5) + H(+); Xref=Rhea:RHEA:56404, Rhea:RHEA-COMP:14464, Rhea:RHEA-COMP:14522, ChEBI:CHEBI:8298, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:140383; EC=3.1.1.75; CATALYTIC ACTIVITY: Reaction=[(3R)-hydroxybutanoate](n) + H2O = (3R)-hydroxybutanoate tetramer + [(3R)-hydroxybutanoate](n-4) + H(+); Xref=Rhea:RHEA:56400, Rhea:RHEA-COMP:14464, Rhea:RHEA-COMP:14521, ChEBI:CHEBI:8298, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:140384; EC=3.1.1.75; |
| DNA Binding | |
| EC Number | 3.1.1.75 |
| Enzyme Function | FUNCTION: This protein degrades water-insoluble and water-soluble PHB to monomeric D(-)-3-hydroxybutyrate. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Chain (1); Domain (1); Signal peptide (1) |
| Keywords | Hydrolase;Lipid degradation;Lipid metabolism;Secreted;Signal |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..27 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 49,935 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:56392; RHEA:56396; RHEA:11248; RHEA:56404; RHEA:56400 |
| Cross Reference Brenda | 3.1.1.75; |