| IED ID | IndEnz0010001642 |
| Enzyme Type ID | esterase001642 |
| Protein Name |
S-formylglutathione hydrolase FGH EC 3.1.2.12 |
| Gene Name | YJL068C HRE299 J1102 |
| Organism | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Saccharomycotina (true yeasts) Saccharomycetes Saccharomycetales Saccharomycetaceae Saccharomyces Saccharomyces cerevisiae (Baker's yeast) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
| Enzyme Sequence | MKVVKEFSVCGGRLIKLSHNSNSTKTSMNVNIYLPKHYYAQDFPRNKRIPTVFYLSGLTCTPDNASEKAFWQFQADKYGFAIVFPDTSPRGDEVANDPEGSWDFGQGAGFYLNATQEPYAQHYQMYDYIHKELPQTLDSHFNKNGDVKLDFLDNVAITGHSMGGYGAICGYLKGYSGKRYKSCSAFAPIVNPSNVPWGQKAFKGYLGEEKAQWEAYDPCLLIKNIRHVGDDRILIHVGDSDPFLEEHLKPELLLEAVKATSWQDYVEIKKVHGFDHSYYFVSTFVPEHAEFHARNLGLI |
| Enzyme Length | 299 |
| Uniprot Accession Number | P40363 |
| Absorption | |
| Active Site | ACT_SITE 161; /note=Charge relay system; /evidence=ECO:0000250|UniProtKB:P10768; ACT_SITE 241; /note=Charge relay system; /evidence=ECO:0000250|UniProtKB:P10768; ACT_SITE 276; /note=Charge relay system; /evidence=ECO:0000250|UniProtKB:P10768 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=H2O + S-formylglutathione = formate + glutathione + H(+); Xref=Rhea:RHEA:14961, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15740, ChEBI:CHEBI:57688, ChEBI:CHEBI:57925; EC=3.1.2.12; Evidence={ECO:0000269|PubMed:10427036}; |
| DNA Binding | |
| EC Number | 3.1.2.12 |
| Enzyme Function | FUNCTION: Serine hydrolase involved in the detoxification of formaldehyde. {ECO:0000269|PubMed:10427036}. |
| Temperature Dependency | BIOPHYSICOCHEMICAL PROPERTIES: Temperature dependence: Optimum temperature is 50 degrees Celsius. {ECO:0000269|PubMed:10427036}; |
| PH Dependency | BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 7. {ECO:0000269|PubMed:10427036}; |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Beta strand (10); Chain (1); Helix (16); Metal binding (2); Turn (2) |
| Keywords | 3D-structure;Copper;Cytoplasm;Direct protein sequencing;Hydrolase;Metal-binding;Reference proteome;Serine esterase |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:10427036}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | X-ray crystallography (4) |
| Cross Reference PDB | 1PV1; 3C6B; 4FLM; 4FOL; |
| Mapped Pubmed ID | 10509023; 19854949; 26760759; 27693354; |
| Motif | |
| Gene Encoded By | |
| Mass | 33,934 |
| Kinetics | |
| Metal Binding | METAL 1; /note="Copper; via amide nitrogen and carbonyl oxygen"; /evidence="ECO:0000269|PubMed:22906720, ECO:0007744|PDB:4FLM"; METAL 140; /note="Copper; via pros nitrogen"; /evidence="ECO:0000269|PubMed:22906720, ECO:0007744|PDB:4FLM" |
| Rhea ID | RHEA:14961 |
| Cross Reference Brenda | 3.1.2.12; |