| IED ID | IndEnz0010001663 |
| Enzyme Type ID | esterase001663 |
| Protein Name |
Temperature shock-inducible protein 1 EC 3.1.1.- |
| Gene Name | TIP1 YBR067C YBR0622 |
| Organism | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Saccharomycotina (true yeasts) Saccharomycetes Saccharomycetales Saccharomycetaceae Saccharomyces Saccharomyces cerevisiae (Baker's yeast) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
| Enzyme Sequence | MSVSKIAFVLSAIASLAVADTSAAETAELQAIIGDINSHLSDYLGLETGNSGFQIPSDVLSVYQQVMTYTDDAYTTLFSELDFDAITKTIVKLPWYTTRLSSEIAAALASVSPASSEAASSSEAASSSKAASSSEATSSAAPSSSAAPSSSAAPSSSAESSSKAVSSSVAPTTSSVSTSTVETASNAGQRVNAGAASFGAVVAGAAALLL |
| Enzyme Length | 210 |
| Uniprot Accession Number | P27654 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.1.1.- |
| Enzyme Function | FUNCTION: Seems to have esterase activity. Prefers ester of fatty acids from 4 to 16 carbon atoms. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Chain (1); Lipidation (1); Propeptide (1); Region (1); Signal peptide (1) |
| Keywords | Cell wall;Direct protein sequencing;GPI-anchor;Glycoprotein;Hydrolase;Lipoprotein;Membrane;Reference proteome;Secreted;Signal;Stress response |
| Interact With | |
| Induction | INDUCTION: Induced during anaerobic growth and by cold shock and heat shock. {ECO:0000269|PubMed:11292809, ECO:0000269|PubMed:7746155}. |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted, cell wall. Membrane; Lipid-anchor, GPI-anchor. Note=Covalently-linked GPI-modified cell wall protein (GPI-CWP) found only in mother cells. |
| Modified Residue | |
| Post Translational Modification | PTM: Extensively O-glycosylated. {ECO:0000269|PubMed:7746155, ECO:0000269|PubMed:7768807}.; PTM: The GPI-anchor is attached to the protein in the endoplasmic reticulum and serves to target the protein to the cell surface. There, the glucosamine-inositol phospholipid moiety is cleaved off and the GPI-modified mannoprotein is covalently attached via its lipidless GPI glycan remnant to the 1,6-beta-glucan of the outer cell wall layer. |
| Signal Peptide | SIGNAL 1..19; /evidence="ECO:0000269|PubMed:10216230, ECO:0000269|PubMed:7746155, ECO:0000269|PubMed:7768807, ECO:0000269|PubMed:9818717" |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 10458981; 11283351; 12683916; 15165243; 15470095; 15896991; 16429126; 16498706; 16554755; 16911045; 17601877; 17910955; 19053375; 19536198; 20193063; 22232249; 22652839; 22835029; 23135325; 25725023; 26113493; 26751950; 27510749; 8127704; 9878836; |
| Motif | |
| Gene Encoded By | |
| Mass | 20,728 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |