| IED ID | IndEnz0010001684 |
| Enzyme Type ID | esterase001684 |
| Protein Name |
2-hydroxy-6-oxo-2,4-heptadienoate hydrolase HOHH EC 3.7.1.25 |
| Gene Name | todF Pput_2882 |
| Organism | Pseudomonas putida (strain ATCC 700007 / DSM 6899 / BCRC 17059 / F1) |
| Taxonomic Lineage | cellular organisms Bacteria Proteobacteria Gammaproteobacteria Pseudomonadales Pseudomonadaceae Pseudomonas Pseudomonas putida group Pseudomonas putida (Arthrobacter siderocapsulatus) Pseudomonas putida (strain ATCC 700007 / DSM 6899 / BCRC 17059 / F1) |
| Enzyme Sequence | MTNVNAEIGRMVLAGGIETNLHDVGAGNPVVLVHGSGPGVTAWANWRTVMPELSRHRRVIAPDMVGFGFTQRPHGIHYGVESWVAHLAGILDALELDRVDLVGNSFGGALSLAFAIRFPHRVRRLVLMGAVGVSFELTDGLDAVWGYEPSVPNMRKVMDYFAYDRSLVSDELAELRYKASTRPGFQEAFASMFPAPRQRWVDALASSDQDIRDIRHETLILHGRDDRVIPLETSLRLNQLIEPSQLHVFGRCGHWVQIEQNRGFIRLVNDFLAAED |
| Enzyme Length | 276 |
| Uniprot Accession Number | P23133 |
| Absorption | |
| Active Site | ACT_SITE 105; /evidence=ECO:0000250; ACT_SITE 226; /evidence=ECO:0000250; ACT_SITE 254; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=(2Z,4E)-2-hydroxy-6-oxohepta-2,4-dienoate + H2O = (2Z)-2-hydroxypenta-2,4-dienoate + acetate + H(+); Xref=Rhea:RHEA:59220, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30089, ChEBI:CHEBI:67152, ChEBI:CHEBI:144490; EC=3.7.1.25; Evidence={ECO:0000305|PubMed:7944378}; |
| DNA Binding | |
| EC Number | 3.7.1.25 |
| Enzyme Function | FUNCTION: Catalyzes the hydrolysis of 2-hydroxy-6-oxohepta-2,4-dienoate into 2-hydroxypenta-2,4-dienoate and acetate. {ECO:0000269|PubMed:1916282, ECO:0000269|PubMed:7944378}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | PATHWAY: Xenobiotic degradation; toluene degradation. |
| nucleotide Binding | |
| Features | Active site (3); Chain (1); Domain (1) |
| Keywords | Aromatic hydrocarbons catabolism;Hydrolase;Serine esterase |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 30,788 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:59220 |
| Cross Reference Brenda |