| IED ID | IndEnz0010001686 |
| Enzyme Type ID | esterase001686 |
| Protein Name |
Uncharacterized esterase Atu5261 EC 3.1.1.- |
| Gene Name | Atu5261 AGR_pAT_374 |
| Organism | Agrobacterium fabrum (strain C58 / ATCC 33970) (Agrobacterium tumefaciens (strain C58)) |
| Taxonomic Lineage | cellular organisms Bacteria Proteobacteria Alphaproteobacteria Hyphomicrobiales Rhizobiaceae Rhizobium/Agrobacterium group Agrobacterium Agrobacterium tumefaciens complex Agrobacterium fabrum Agrobacterium fabrum (strain C58 / ATCC 33970) (Agrobacterium tumefaciens (strain C58)) |
| Enzyme Sequence | MEDIMTSLLVATSRVVVTISLAYVPVKSAFAAPQNAAGLCSAIAASSIPASRISLPTTGAKIQSATLIGGSDPANQNGEYCKVIGQIKPVDPKAPDIIWQVNLPSVWNGKMLQYGGGGYNGSIPPTTDKTTLGLDVVPTPLTQGYVTFGSDSGHQAPNADDASFAKNDEAMLNYGYMHIKKVLDVAKVLVTERYEKPVTRVYFQGGSTGGREGLTAASRWPESYDGILTNYPTANFVGLRLWGAGLARAVYDDKSAGWIPPKLVERISKEALKSCDGLDGVEDGLVGNMQQCRAQSAVLVQSLACKSDVTGNPDDCLTQAQIERTLKIYHEGYSLPYQLANGINTYPGYNSLEGIMMQLGSEPQMRTPPVSGPNAHHSSRSFEFLQNFVQRDQPLNLLSFDIREPGKLKDRIVELSDVIGATRTDWSTFSDRGGKIIWLQGNDDPSVSPLGNAKLFESIVAKMGADKVKGFMRFFLVPGLAHGGGRFSPTWDNLAALDNWVEHDVPPSNPVVVDATKSSTKGRSRPLCEYPSWPKYKGDGDVAIASSFSCADD |
| Enzyme Length | 553 |
| Uniprot Accession Number | Q8UK62 |
| Absorption | |
| Active Site | ACT_SITE 207; /note=Acyl-ester intermediate; /evidence=ECO:0000250|UniProtKB:Q2UP89; ACT_SITE 444; /note=Charge relay system; /evidence=ECO:0000250|UniProtKB:Q2UP89; ACT_SITE 482; /note=Charge relay system; /evidence=ECO:0000250|UniProtKB:Q2UP89 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.1.1.- |
| Enzyme Function | |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Chain (1); Disulfide bond (2); Erroneous initiation (1); Metal binding (5); Signal peptide (1) |
| Keywords | Calcium;Disulfide bond;Hydrolase;Metal-binding;Plasmid;Reference proteome;Serine esterase;Signal |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..31; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | Plasmid AT |
| Mass | 59,523 |
| Kinetics | |
| Metal Binding | METAL 276; /note=Calcium; /evidence=ECO:0000250|UniProtKB:Q2UP89; METAL 279; /note=Calcium; /evidence=ECO:0000250|UniProtKB:Q2UP89; METAL 281; /note=Calcium; via carbonyl oxygen; /evidence=ECO:0000250|UniProtKB:Q2UP89; METAL 283; /note=Calcium; /evidence=ECO:0000250|UniProtKB:Q2UP89; METAL 285; /note=Calcium; via carbonyl oxygen; /evidence=ECO:0000250|UniProtKB:Q2UP89 |
| Rhea ID | |
| Cross Reference Brenda |