| IED ID | IndEnz0010001693 |
| Enzyme Type ID | esterase001693 |
| Protein Name |
Sterol esterase 2 EC 3.1.1.13 Steryl ester hydrolase 2 |
| Gene Name | YEH2 YLR020C |
| Organism | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Saccharomycotina (true yeasts) Saccharomycetes Saccharomycetales Saccharomycetaceae Saccharomyces Saccharomyces cerevisiae (Baker's yeast) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
| Enzyme Sequence | MVNKVVDEVQRLVSAIILTSFMTGLFILSLWKNYVTVHFQHKNDPRDTRSSRTKIQPNDKKKKRPARHSRPLSISSTTPLDLQRDQENNIEYDRTVTSKLSMTSNASLSENGDGNANIKMETNVNQAPYAAENPFQNIALAEDTKLVPDLKYYYKEYGIDIEEFEVETDDGFIIDLWHFKSRLNDGVEEVKREPILLLHGLLQSCGAFASSGRKSLAYFLYESGFDVWLGNNRCGLNAKWNMKKLGNDHSKKWDWDMHQMVQYDLKALINYVLDSTGYAKLSLVAHSQGTTQGFMGLVNGEKLYASDFKLVDKLENFVALAPAVYPGPLLDEKAFVRLMAKGIDSPWYFGRRSFIPLMMTMRKLMVGTKIFSFLSYIMFNYLFDWNDVLWDRVLRDRNFLFSPVHISVKLMQWWLSPLPNKLSFKKGAEKIFPDKKTWFPIAKNDDDSGNNLDNNKLHLNPKRQNSEEFPHIIMFIPKQDRLVDGERLINHFINHEANAVYKIWYIDEYSHLDVLWAHDVIDRIGKPMIENLRFPNAR |
| Enzyme Length | 538 |
| Uniprot Accession Number | Q07950 |
| Absorption | |
| Active Site | ACT_SITE 287; /note=Nucleophile; /evidence=ECO:0000250; ACT_SITE 480; /note=Charge relay system; /evidence=ECO:0000250; ACT_SITE 511; /note=Charge relay system; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=a sterol ester + H2O = a fatty acid + a sterol + H(+); Xref=Rhea:RHEA:10100, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15889, ChEBI:CHEBI:28868, ChEBI:CHEBI:35915; EC=3.1.1.13; Evidence={ECO:0000269|PubMed:15713625}; |
| DNA Binding | |
| EC Number | 3.1.1.13 |
| Enzyme Function | FUNCTION: Mediates the hydrolysis of steryl esters. Required for mobilization of steryl ester, thereby playing a central role in lipid metabolism. {ECO:0000269|PubMed:15632184, ECO:0000269|PubMed:15713625}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Chain (1); Compositional bias (1); Modified residue (2); Region (1); Topological domain (2); Transmembrane (1) |
| Keywords | Cell membrane;Hydrolase;Lipid degradation;Lipid metabolism;Membrane;Phosphoprotein;Reference proteome;Signal-anchor;Transmembrane;Transmembrane helix |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:15632184, ECO:0000269|PubMed:15713625}; Single-pass type II membrane protein {ECO:0000269|PubMed:15632184, ECO:0000269|PubMed:15713625}. |
| Modified Residue | MOD_RES 73; /note="Phosphoserine"; /evidence="ECO:0007744|PubMed:17330950, ECO:0007744|PubMed:18407956, ECO:0007744|PubMed:19779198"; MOD_RES 107; /note="Phosphoserine"; /evidence="ECO:0007744|PubMed:19779198" |
| Post Translational Modification | PTM: Not glycosylated. |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 14587103; 14690591; 16246075; 16835446; 16919863; 17434796; 18258205; 18269180; 18467557; 19111628; 19536198; 21621788; 22194828; 22345606; 23139841; 23202731; 23275493; 24520995; 24597968; 25720564; 25894691; 26162625; 26636650; 28772; |
| Motif | |
| Gene Encoded By | |
| Mass | 62,447 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:10100 |
| Cross Reference Brenda | 3.1.1.13; |