| IED ID | IndEnz0010001707 |
| Enzyme Type ID | esterase001707 |
| Protein Name |
Neuropathy target esterase sws Swiss cheese EC 3.1.1.5 |
| Gene Name | sws GH24540 |
| Organism | Drosophila grimshawi (Hawaiian fruit fly) (Idiomyia grimshawi) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Protostomia Ecdysozoa Panarthropoda Arthropoda Mandibulata Pancrustacea Hexapoda Insecta Dicondylia Pterygota (winged insects) Neoptera Endopterygota Diptera Brachycera Muscomorpha Eremoneura Cyclorrhapha Schizophora Acalyptratae Ephydroidea Drosophilidae (pomace flies) Drosophilinae Drosophilini Drosophila (fruit flies) Hawaiian Drosophila picture wing clade grimshawi clade grimshawi group grimshawi subgroup Drosophila grimshawi (Hawaiian fruit fly) (Idiomyia grimshawi) |
| Enzyme Sequence | MDVLELLRASATGCYNTIFSEAWHQYVHKQIAAAVYWYGALFLLGVLLFVWFLYFKRLARLRLRDEIARSLSAVTSAATGVDHRGLRFRKRDKMLFYGRRMLRKMKNVSGQMYSSGKGYKRRAVMRFARRILQLQRENRPLEMKTVEPPAEYLEETIEGSDRVPPDALYMLQSIRIFGHFEKPIFLKLCKHTQILQLMAGDYLFKITDPDDSVYIVQSGMINVYICNADGSTLSLKTVRKGESVTSLLSFIDVLSGNSSYYKTVTAKAIEKSVVIRLPMQAFEEVFDENPDVMIRVIQVIMIRLQRVLFTALRNYLGLNAELVQNHMRNKNSSNPMATGQTTSNVQSQTSQATQSRPSGTTRTPTSPMLRLSREEHTLSDPDPNPNANNLHYHEMHGDAPYIDLYHYQQQQQSLNSPRRNSTAHVSEAAAASTASSPTTIDQRLVQSSAVDSLRRELGLGEEDAHIIEQFVLVRELEPNVTLITEGNADDVCIWFVMTGNLAVYQSNADATRASAKQDAKPEMLIHFVHPGEIVGGLAMLTGEASAYTIRSRNNSRVAFIRRAAIYQIMRQRPRIVLGLGNGVVRRLSPLVRQCDYALDWIFLESGRAVYRQDESSDSTYIVLSGRMRSVITQPNGKKEIVGEYGKGDLVGIVEMITETSRTTTVMAVRDSELAKLPEGLFNAIKLRYPIVVTRLISFLSHRFLGSMQTRGSNAYGTPVEANPVTHKYSTVALVPITDDVPLTPFTYELYHSLCAIGPVLRLTSEVVGKQLGVNIFDTANEYRLTSWLAQQEDRNIITLYQCDSSLSPWTQRCMRQADVILIVGLGERSHLVGKFEREIDKLAMRTQKELVLLYPETTNAKPANTLSWLNARPWVTKHHHVLCVKRIFTRKSQYRINDLYSRVLLSEPNMHSDFSRLARWLTGNSIGLVLGGGGARGAAHIGMLKAIQEAGIPIDMVGGVSIGALMGALWCSERNITTVTQKAREWSKKMTKWFLQLLDLTYPITSMFSGREFNKTIHDTFGDVTIEDLWIPYFTLTTDITASCHRIHTNGSLWRFVRSSMSLSGYMPPLCDPQDGHLLLDGGYVNNLPGHLWRYCRASMSIAGVFPPFCDYRDGHLLLDGCYTNNVPADVMHNLGAAHIIAIDVGSQDDTDLTNYGDDLSGWWLLYKKWNPFTSPVKVPDLPDIQSRLAYVSCVRQLEEVKNSDYCEYIRPPIDKYKTLAFGSFDEIRDVGYVFGKNYFDTMAKAGRLGRFNQWFNKEPPKRGNHASLNEYTFIDLAQIVCKLPETYVRLGTPDLFSEDEDEDFDGYISEPTTLNTDRRRIQVHRAGNSLSFSEAELDSDVELDLEMENKVDKATQSTPTLPDKRSVQTPTPSLFNLTMPIAVDEIDKSSGRVKRKLEATNTASIAEPEASPSIKAEATTQTTPPTSKRTEQDEHELEHEQVVEKQQVMDKQQGNTTNNDTKN |
| Enzyme Length | 1464 |
| Uniprot Accession Number | B4JLX2 |
| Absorption | |
| Active Site | ACT_SITE 961; /note=Nucleophile; /evidence=ECO:0000255|PROSITE-ProRule:PRU01161; ACT_SITE 1081; /note=Proton acceptor; /evidence=ECO:0000255|PROSITE-ProRule:PRU01161 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=a 1-acyl-sn-glycero-3-phosphocholine + H2O = a fatty acid + H(+) + sn-glycerol 3-phosphocholine; Xref=Rhea:RHEA:15177, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:16870, ChEBI:CHEBI:28868, ChEBI:CHEBI:58168; EC=3.1.1.5; Evidence={ECO:0000250|UniProtKB:Q9U969}; |
| DNA Binding | |
| EC Number | 3.1.1.5 |
| Enzyme Function | FUNCTION: Phospholipase B that deacylates intracellular phosphatidylcholine (PtdCho), generating glycerophosphocholine (GroPtdCho). This deacylation occurs at both sn-2 and sn-1 positions of PtdCho. Its specific chemical modification by certain organophosphorus (OP) compounds leads to distal axonopathy. Plays a role in the signaling mechanism between neurons and glia that regulates glia wrapping during development of the adult brain. Essential for membrane lipid homeostasis and cell survival in both neurons and glia of the adult brain (By similarity). {ECO:0000250|UniProtKB:Q9U969}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | NP_BIND 176..303; /note=cNMP 1; /evidence=ECO:0000255; NP_BIND 456..586; /note=cNMP 2; /evidence=ECO:0000255; NP_BIND 575..702; /note=cNMP 3; /evidence=ECO:0000255 |
| Features | Active site (2); Chain (1); Compositional bias (5); Domain (1); Modified residue (2); Motif (3); Nucleotide binding (3); Region (4); Topological domain (2); Transmembrane (1) |
| Keywords | Developmental protein;Endoplasmic reticulum;Hydrolase;Lipid degradation;Lipid metabolism;Membrane;Neurogenesis;Phosphoprotein;Reference proteome;Transmembrane;Transmembrane helix |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000250|UniProtKB:Q9U969}; Single-pass type I membrane protein {ECO:0000250|UniProtKB:Q9U969}. Note=Sws tethers Pka-C3 to the membrane. {ECO:0000250|UniProtKB:Q9U969}. |
| Modified Residue | MOD_RES 421; /note=Phosphoserine; /evidence=ECO:0000250|UniProtKB:Q9U969; MOD_RES 1175; /note=Phosphoserine; /evidence=ECO:0000250|UniProtKB:Q9U969 |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | MOTIF 932..937; /note=GXGXXG; /evidence=ECO:0000255|PROSITE-ProRule:PRU01161; MOTIF 959..963; /note=GXSXG; /evidence=ECO:0000255|PROSITE-ProRule:PRU01161; MOTIF 1081..1083; /note=DGA/G; /evidence=ECO:0000255|PROSITE-ProRule:PRU01161 |
| Gene Encoded By | |
| Mass | 165,239 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:15177 |
| Cross Reference Brenda |