Detail Information for IndEnz0010001727
IED ID IndEnz0010001727
Enzyme Type ID esterase001727
Protein Name S-formylglutathione hydrolase FrmB
FGH
EC 3.1.2.12
Gene Name frmB ECDH10B_0310
Organism Escherichia coli (strain K12 / DH10B)
Taxonomic Lineage cellular organisms Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Enterobacteriaceae Escherichia Escherichia coli Escherichia coli (strain K12) Escherichia coli (strain K12 / DH10B)
Enzyme Sequence MELIEKHVSFGGWQNMYRHYSQSLKCEMNVGVYLPPKAANEKLPVLYWLSGLTCNEQNFITKSGMQRYAAEHNIIVVAPDTSPRGSHVADADRYDLGQGAGFYLNATQAPWNEHYKMYDYIRNELPDLVMHHFPATAKKSISGHSMGGLGALVLALRNPDEYVSVSAFSPIVSPSQVPWGQQAFAAYLAENKDAWLDYDPVSLISQGQRVAEIMVDQGLSDDFYAEQLRTPNLEKICQEMNIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNMR
Enzyme Length 277
Uniprot Accession Number B1XEU8
Absorption
Active Site ACT_SITE 145; /note=Charge relay system; /evidence=ECO:0000250; ACT_SITE 221; /note=Charge relay system; /evidence=ECO:0000250; ACT_SITE 254; /note=Charge relay system; /evidence=ECO:0000250
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=H2O + S-formylglutathione = formate + glutathione + H(+); Xref=Rhea:RHEA:14961, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15740, ChEBI:CHEBI:57688, ChEBI:CHEBI:57925; EC=3.1.2.12;
DNA Binding
EC Number 3.1.2.12
Enzyme Function FUNCTION: Serine hydrolase involved in the detoxification of formaldehyde. Hydrolyzes S-formylglutathione to glutathione and formate (By similarity). {ECO:0000250}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (3); Chain (1)
Keywords Hydrolase;Serine esterase
Interact With
Induction
Subcellular Location
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 31,424
Kinetics
Metal Binding
Rhea ID RHEA:14961
Cross Reference Brenda