Detail Information for IndEnz0010001736
IED ID IndEnz0010001736
Enzyme Type ID esterase001736
Protein Name 2-hydroxymuconate semialdehyde hydrolase
HMSH
EC 3.7.1.9
2-hydroxymuconic semialdehyde hydrolase
Gene Name xylF
Organism Pseudomonas putida (Arthrobacter siderocapsulatus)
Taxonomic Lineage cellular organisms Bacteria Proteobacteria Gammaproteobacteria Pseudomonadales Pseudomonadaceae Pseudomonas Pseudomonas putida group Pseudomonas putida (Arthrobacter siderocapsulatus)
Enzyme Sequence MNAPQQSPEIGREILAAGYRTNLHDQGEGFPALLIHGSGPASPPGPTGAGSFRSSQTRRVIAPDMLGFGYSERPADGKYSQARWVEHAIGVLDALGIQQGDIVGNSFGGGLALALAIRHPERVRRLVLMGSVGVSFPITAGLETAWGYTPSLANMRRLLDLFAHDRTLVNDELAELRYQASIRPGFQESFAAMFPPPRQNGVDDLASNETDIRALPNETLVIHGREDRIIPLQASLTLAQWIPNAQLHVFGQCGHWTQIEHAERFARLVENFLAEADALHS
Enzyme Length 281
Uniprot Accession Number P23106
Absorption
Active Site ACT_SITE 106; /evidence=ECO:0000250; ACT_SITE 227; /evidence=ECO:0000250; ACT_SITE 255; /evidence=ECO:0000250
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=(2Z,4E)-2-hydroxy-6-oxohexa-2,4-dienoate + H2O = 2-oxopent-4-enoate + formate + H(+); Xref=Rhea:RHEA:14549, ChEBI:CHEBI:11641, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15740, ChEBI:CHEBI:71198; EC=3.7.1.9; Evidence={ECO:0000269|Ref.2};
DNA Binding
EC Number 3.7.1.9
Enzyme Function FUNCTION: Catalyzes the conversion of 2-hydroxymuconate semialdehyde to 2-hydroxypent-2,4-dienoate. {ECO:0000269|Ref.2}.
Temperature Dependency
PH Dependency BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 7-7.5. {ECO:0000269|Ref.2};
Pathway PATHWAY: Aromatic compound metabolism; benzoate degradation via hydroxylation.
nucleotide Binding
Features Active site (3); Chain (1); Domain (1); Region (1)
Keywords Aromatic hydrocarbons catabolism;Direct protein sequencing;Hydrolase;Plasmid;Serine esterase
Interact With
Induction
Subcellular Location
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By Plasmid TOL pWW0
Mass 30,598
Kinetics BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=30 uM for catechol {ECO:0000269|Ref.2}; KM=36 uM for 3-methylcatechol {ECO:0000269|Ref.2}; KM=10 uM for 4-methylcatechol {ECO:0000269|Ref.2}; KM=15 uM for 3-ethylcatechol {ECO:0000269|Ref.2}; KM=22 uM for 4-ethylcatechol {ECO:0000269|Ref.2}; KM=5 uM for 3-propylcatechol {ECO:0000269|Ref.2}; KM=23 uM for 4-propylcatechol {ECO:0000269|Ref.2}; KM=37 uM for 3-allylcatechol {ECO:0000269|Ref.2};
Metal Binding
Rhea ID RHEA:14549
Cross Reference Brenda