Detail Information for IndEnz0010001749
IED ID IndEnz0010001749
Enzyme Type ID esterase001749
Protein Name Pyrethroid hydrolase
EC 3.1.1.88
Gene Name estP
Organism Klebsiella sp.
Taxonomic Lineage cellular organisms Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Enterobacteriaceae Klebsiella/Raoultella group Klebsiella unclassified Klebsiella Klebsiella sp.
Enzyme Sequence MEICTKGSRKHLTSRASEPSYNVPENQYVLYVVSSTLSIVKQLVKVAESKKSRFSGAIEKLNERLDSLKDYRIINRDLVVKDLERLKKRFDTEVINAELSEQLAKINVNLSRSYSEKGYLRLEKATGSENEWWAKIKPSQNDDWQQFEPDGYTIFSSRDHYASLFKSYSDYEIEAKIPLPLRRGKAVVLYPEYISRICVLPESRSIQREQENFTKLRDKGIALSKKDWQAKLTTDELAEQEKERATINKRLGYFATEHEKVGIVHKALEPKLKPFQQIEKEWRQCKVKSKSTFPNSMTFVQNPAYQAVHSGFKKLKEQIGLADEDILLSLEKIEAIGLVNMPLLYERWCLLQIIKVLTQAFRYQPEDNWKRKLIANIQGNEEQISIQFFNPSVSRAITLQYEPFLANGKRPDFVLDVEAITKSGNQISKRLVVDAKYYSAAYLKQRGGIGGVIHELYNGKDYSECQENSVFVLHPVLDAVEKVVSPQEWAKDSYLGELSMFDWEPAHHQRQATNYGAVCANPMKSQRYLDEIQRMLGMFLQYGIEDNTSFRGASDDTHAVNFCVSCGSEKVVDVTKSMSSNNQKRWYRCNECTHFTVYTHCGTCNTRLIKNGEYWTYLSLMPMSSINIKCPNCESPV
Enzyme Length 637
Uniprot Accession Number Q52NW7
Absorption
Active Site
Activity Regulation ACTIVITY REGULATION: Inhibited by Hg(2+), Ag(+) and rho-chloromercuribenzoate. {ECO:0000269|PubMed:16448191}.
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=(-)-trans-permethrin + H2O = (1S,3R)-3-(2,2-dichlorovinyl)-2,2-dimethylcyclopropanecarboxylate + (3-phenoxyphenyl)methanol + H(+); Xref=Rhea:RHEA:30283, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:62523, ChEBI:CHEBI:62527, ChEBI:CHEBI:62531; EC=3.1.1.88; Evidence={ECO:0000269|PubMed:16448191};
DNA Binding
EC Number 3.1.1.88
Enzyme Function FUNCTION: Catalyzes the hydrolysis of pyrethroids pesticides. Hydrolyzes cis-permethrin at approximately equal rate to trans-permethrin. {ECO:0000269|PubMed:16448191}.
Temperature Dependency
PH Dependency BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 7.0. {ECO:0000269|PubMed:16448191};
Pathway
nucleotide Binding
Features Chain (1)
Keywords Direct protein sequencing;Hydrolase;Serine esterase
Interact With
Induction
Subcellular Location
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 73,427
Kinetics BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=0.16 uM for cis-permethrin {ECO:0000269|PubMed:16448191}; KM=0.11 uM for trans-permethrin {ECO:0000269|PubMed:16448191}; KM=0.21 uM for cypermethrin {ECO:0000269|PubMed:16448191}; KM=0.91 uM for fenvalerate {ECO:0000269|PubMed:16448191}; KM=1.23 uM for deltamethrin {ECO:0000269|PubMed:16448191}; KM=0.87 uM for malathion {ECO:0000269|PubMed:16448191}; Note=kcat is 1.05 sec(-1) with cis-permethrin as substrate. kcat is 1.14 sec(-1) with trans-permethrin as substrate. kcat is 0.288 sec(-1) with cypermethrin as substrate. kcat is 0.103 sec(-1) with fenvalerate as substrate. kcat is 0.016 sec(-1) with deltamethrin as substrate. kcat is 0.07 sec(-1) with malathion as substrate.;
Metal Binding
Rhea ID RHEA:30283
Cross Reference Brenda 3.1.1.1;