| IED ID | IndEnz0010001774 |
| Enzyme Type ID | esterase001774 |
| Protein Name |
Xyloglucan O-acetyltransferase 3 PtrXGOAT3 EC 2.3.1.- |
| Gene Name | XGOAT3 POPTR_011G144100 |
| Organism | Populus trichocarpa (Western balsam poplar) (Populus balsamifera subsp. trichocarpa) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids fabids Malpighiales Salicaceae Saliceae Populus (poplars) Populus trichocarpa (Western balsam poplar) (Populus balsamifera subsp. trichocarpa) |
| Enzyme Sequence | MNRFFYTVGLIFLFSFFILYSPKTSDLSNNVDLHQQLLISLQKEEERCDLFSGYWVQDLRGSQYTNVSCSSIPESKNCFMQGRPDAGFSQWRWKPDGCELPRFDPGTFFEIVRGKTMAFIGDSVARNHVESLLCLLSSEEMPLGIYKDTEDRTRTWYFPHSNFTLMVIWTRFLVLDEERVINGSVTGVFDLHLDKMDKNWANKLPEIDYAILSDAHWFFRKNYLYEKGKNIGCIFCGEPGIKSLDIDSALQMVIKVVLNYINNCKKCRNILTVLRTFSPAHFADGAWDTGGSCNRTHPLGEKEIDLASLDWKIRSIQVEEIKRVRPVARRRKKFEVLDVTKAMLMRPDGHPNSYWGNKWMKGYNDCVHWCMPGPIDAWNDFLIALLRRHAFTDFTWS |
| Enzyme Length | 397 |
| Uniprot Accession Number | A0A2K1YKA4 |
| Absorption | |
| Active Site | ACT_SITE 123; /note=Nucleophile; /evidence=ECO:0000250|UniProtKB:Q9LY46; ACT_SITE 365; /note=Proton donor; /evidence=ECO:0000250|UniProtKB:Q9LY46; ACT_SITE 368; /note=Proton acceptor; /evidence=ECO:0000250|UniProtKB:Q9LY46 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 2.3.1.- |
| Enzyme Function | FUNCTION: Xyloglucan acetyltransferase that catalyzes the acetylation of fucosylated Gal residues on xyloglucan side chains (PubMed:30083810). Predominantly catalyze 6-O-monoacetylation of Gal residues in the Fuc-Gal-Xyl trisaccharide side chains of xyloglucan oligomers (PubMed:30083810). {ECO:0000269|PubMed:30083810}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Chain (1); Disulfide bond (4); Glycosylation (4); Motif (2); Topological domain (2); Transmembrane (1) |
| Keywords | Disulfide bond;Glycoprotein;Golgi apparatus;Membrane;Reference proteome;Signal-anchor;Transferase;Transmembrane;Transmembrane helix |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Golgi apparatus membrane {ECO:0000305}; Single-pass type II membrane protein {ECO:0000305}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | MOTIF 121..123; /note=GDS motif; /evidence=ECO:0000305|PubMed:30083810; MOTIF 365..368; /note=DXXH motif; /evidence=ECO:0000305|PubMed:30083810 |
| Gene Encoded By | |
| Mass | 46,209 |
| Kinetics | BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=2.48 mM for xyloglucan oligomer {ECO:0000269|PubMed:30083810}; Vmax=23.1 pmol/min/mg enzyme with xyloglucan oligomer as substrate {ECO:0000269|PubMed:30083810}; |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |