| IED ID | IndEnz0010001792 |
| Enzyme Type ID | esterase001792 |
| Protein Name |
Lysophospholipase NTE1 EC 3.1.1.5 Intracellular phospholipase B Neuropathy target esterase homolog |
| Gene Name | NTE1 AER124W |
| Organism | Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056) (Yeast) (Eremothecium gossypii) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Saccharomycotina (true yeasts) Saccharomycetes Saccharomycetales Saccharomycetaceae Eremothecium Eremothecium gossypii (Yeast) (Ashbya gossypii) Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056) (Yeast) (Eremothecium gossypii) |
| Enzyme Sequence | MVDGTYVNSSVEAISGEADTAAVAELANQMVMNFLYESSSWLAQSTGPFLWKGFRFLFLRLPSSLLVYLINGTVPFYLVVLGSVFTPIIVYLILRSRVLSAYSRLKGDNEDEVIDKKKQLVNDSEAMLMGGPDGKRRSGFSSYLDEFLSAIKIFGYLDKLVFHELTKSMRTQRLEQGEVMLLDDSVGFAIVVEGGLHIYHKIAHSQGSTRESMPLPEDRSFDSNEDDLTINGERFQLLNKVKAGNPVSSLVSILKLFTDNHTPTVVDSSKSSPKQSAQVPIRQLISPFLDGNVERNKATLNMKQVPTAEYEIPSAERAGSNVSSFTQQLYESMLPKDHAAEDVREPLPEIVAYAASDCAIAVIPASSFKRLMVKYPRSASQIIQVILTKLYRVTFQTAHSYLGLTKEIMHTEVVLNNSTNFELPYYLQEAILRKIKNGSKESSVQESIHRATLRSKNVHAHNSPTPNKISRHIALESRDQYNPGDLLSNVPLSRKGSKTASSSSVSIPRISSLRHQKEAASSPLATLRRLEGNDSQNFAPLSSSSPMSVSEKPSVVGGGAHDNISQISFSSALEETEESSWRMALVESMFGYLGITNESIMPPTEDLLFLNNRASSGSSVCSVSSYSHPQTINQDLFRCLSPELVRTKKKAPRQKYTEEMPINVDFNTAKEEFAEGLETLFIPSGATIVEQNGNNKGLYYIVSGELLVCWKNEEDNIEYVLYTVKPGGIAGYLASLIGFKSFVSLRAKTDLYVGFLPIEVLERLCDKYFMIYLKIAETLTKLLSPKILKLDYALEWIHLEASETLFNQNDPANAIYVVLNGRLRQLHQKSKNEERLSRPTTQRKKRKDDNQPNVQVVGEYSQGCSFGEVEVLTAMNRVSTVVAVRDTELARIPRTLFEVLALEHPSIMIRVSRLVAHKILQRSSDIREPTKIVNSANGYRYDFNLTIPPSAGTSSWGNNSDGGSISYKTITILPITYGLPVEEFANKLVSTFRQVGRSTIGLTQCTTLKHLGRHAFDKLANLKQSGYFAELEELYELVVYIADTPVKSSWTSTCISQGDCILLLADASSDPEIGEFERLLINNRTTARTELLLLHPERYVEPGLTHKWLRKRTWVHQHHHMQFVSSQNPSERVDSKAPPIPGAPPNLIGRLKKRERLNQLTKRTQENFARLLPDSIKLTVENISMKYIQKKQKYYTPVSAHKNDFLRLARILSGQAIGLVLGGGGARGISHLGILKAIEEHGIPIDMIGGTSIGSFVGGLYAKDYDLVPTYGRVKKFAGRIGSIWRMLSDLTWPVTSYTTGHEFNRGIWKAFGDIRIEDFWIQYYCNSTNITESMQEIHTFGYAWRYVRASMSLAGILPPITDNGNMLLDGGYLDNLPVLEMKARGCKTIFAVDVGSVDDRTPMDYGDSLNGFWIVLNRWNPFSKHPNVPSMAEIQMRLGYVASVNALEKAKTTQGVVYFRPPIEDYATLDFAKFEEIYQVGTAYGATFLHELEQNGKLPRIPGNEPANGPGIHLLHRRNSI |
| Enzyme Length | 1522 |
| Uniprot Accession Number | Q756Z0 |
| Absorption | |
| Active Site | ACT_SITE 1252; /note=Nucleophile; /evidence=ECO:0000255|PROSITE-ProRule:PRU01161; ACT_SITE 1370; /note=Proton acceptor; /evidence=ECO:0000255|PROSITE-ProRule:PRU01161 |
| Activity Regulation | ACTIVITY REGULATION: Inhibited by organophosphorus esters. {ECO:0000250}. |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=a 1-acyl-sn-glycero-3-phosphocholine + H2O = a fatty acid + H(+) + sn-glycerol 3-phosphocholine; Xref=Rhea:RHEA:15177, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:16870, ChEBI:CHEBI:28868, ChEBI:CHEBI:58168; EC=3.1.1.5; |
| DNA Binding | |
| EC Number | 3.1.1.5 |
| Enzyme Function | FUNCTION: Intracellular phospholipase B that catalyzes the double deacylation of phosphatidylcholine (PC) to glycerophosphocholine (GroPCho). Plays an important role in membrane lipid homeostasis. Responsible for the rapid PC turnover in response to inositol, elevated temperatures, or when choline is present in the growth medium (By similarity). {ECO:0000250}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | NP_BIND 661..782; /note=cNMP 1; NP_BIND 778..918; /note=cNMP 2 |
| Features | Active site (2); Chain (1); Compositional bias (2); Domain (1); Motif (3); Nucleotide binding (2); Region (5); Topological domain (2); Transmembrane (1) |
| Keywords | Endoplasmic reticulum;Hydrolase;Lipid degradation;Lipid metabolism;Membrane;Reference proteome;Repeat;Transmembrane;Transmembrane helix |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000305}; Single-pass type I membrane protein {ECO:0000305}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | MOTIF 1223..1228; /note=GXGXXG; /evidence=ECO:0000255|PROSITE-ProRule:PRU01161; MOTIF 1250..1254; /note=GXSXG; /evidence=ECO:0000255|PROSITE-ProRule:PRU01161; MOTIF 1370..1372; /note=DGA/G; /evidence=ECO:0000255|PROSITE-ProRule:PRU01161 |
| Gene Encoded By | |
| Mass | 170,071 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:15177 |
| Cross Reference Brenda |