Detail Information for IndEnz0010001804
IED ID IndEnz0010001804
Enzyme Type ID esterase001804
Protein Name Ubiquitin-conjugating enzyme E2-18 kDa
EC 2.3.2.23
E2 ubiquitin-conjugating enzyme 84D
Ubiquitin carrier protein
Ubiquitin-protein ligase
Gene Name Ubc84D CG12799
Organism Drosophila melanogaster (Fruit fly)
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Protostomia Ecdysozoa Panarthropoda Arthropoda Mandibulata Pancrustacea Hexapoda Insecta Dicondylia Pterygota (winged insects) Neoptera Endopterygota Diptera Brachycera Muscomorpha Eremoneura Cyclorrhapha Schizophora Acalyptratae Ephydroidea Drosophilidae (pomace flies) Drosophilinae Drosophilini Drosophila (fruit flies) Sophophora melanogaster group melanogaster subgroup Drosophila melanogaster (Fruit fly)
Enzyme Sequence MAATRRLTRELSDLVEAKMSTLRNIESSDESLLMWTGLLVPEKAPYNKGAFRIEINFPPQYPFMPPKILFKTKIYHPNVDEKGEVCLPIISTDNWKPTTRTEQVLQALVAIVHNPEPEHPLRSDLAEEFVREHKKFMKTAEEFTKKNAEKRPE
Enzyme Length 153
Uniprot Accession Number P52487
Absorption
Active Site ACT_SITE 86; /note="Glycyl thioester intermediate"; /evidence="ECO:0000255|PROSITE-ProRule:PRU00388, ECO:0000255|PROSITE-ProRule:PRU10133"
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.; EC=2.3.2.23; Evidence={ECO:0000255|PROSITE-ProRule:PRU00388, ECO:0000255|PROSITE-ProRule:PRU10133};
DNA Binding
EC Number 2.3.2.23
Enzyme Function FUNCTION: Catalyzes the covalent attachment of ubiquitin to other proteins. {ECO:0000255|PROSITE-ProRule:PRU00388}.
Temperature Dependency
PH Dependency
Pathway PATHWAY: Protein modification; protein ubiquitination. {ECO:0000255|PROSITE-ProRule:PRU00388}.
nucleotide Binding
Features Active site (1); Chain (1); Domain (1)
Keywords ATP-binding;Nucleotide-binding;Reference proteome;Transferase;Ubl conjugation pathway
Interact With
Induction
Subcellular Location
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID 10742048; 10948271; 14605208; 19452197; 20220848; 20371351; 20617381; 21074052; 22494833; 22562043; 23071443; 25312911; 26707646; 31722958;
Motif
Gene Encoded By
Mass 17,746
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda