Detail Information for IndEnz0010001809
IED ID IndEnz0010001809
Enzyme Type ID esterase001809
Protein Name Xylan O-acetyltransferase 12
EC 2.3.1.-
Protein trichome birefringence-like 2
OsTBL2
Gene Name XOAT12 TBL2 Os11g0107000 LOC_Os11g01570 OsJ_32659
Organism Oryza sativa subsp. japonica (Rice)
Taxonomic Lineage cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae Liliopsida Petrosaviidae commelinids Poales Poaceae BOP clade Oryzoideae Oryzeae Oryzinae Oryza Oryza sativa (Rice) Oryza sativa subsp. japonica (Rice)
Enzyme Sequence MWSALFSHLREVHKRSGVKEEKLIMKSPAAAGEAAGCHKPQATATNKMTVLQSPLGLRTILTSLVAFFIVVSSVSLLFDRSQDAQAQLAVAQHQHQEVQLKQKPASAAVGEQKSVFVDQSSLRSQEAQVQWTSELQDVATDSGDGGVDGEEECNWSLGRWVYDNSSRPLYSGLKCSFIFDEVACDKYGRNDTKYQHWRWQPHGCNLPRFNATKFLEKLRNKRLVFVGDSVNRNQWVSMVCMVEHFIPDGRKMRVYNGSLISFKAFEYNATIDFYWSPLLLESNSDNPIIHRVEYRIIRADRIEKHANVWKDADFIVFNSYLWWRKQRDGMTMKVMYGSFEDGDAKLDEVEMVDGYEIALKKLTEYLGANINKNKTRIFFAGSSPAHSWASNWGGDDNNKCLNETEPIQIEDYRSATTDYGMMDKAKEIFGTLEPKGIHVQILNITQLSEYRKDAHPTIFRRQYVPLTKEQIANPSIYADCTHWCLPGVPDVWNEFLYAYLMHK
Enzyme Length 503
Uniprot Accession Number Q2RBL8
Absorption
Active Site ACT_SITE 229; /note=Nucleophile; /evidence=ECO:0000250|UniProtKB:Q9LY46; ACT_SITE 479; /note=Proton donor; /evidence=ECO:0000250|UniProtKB:Q9LY46; ACT_SITE 482; /note=Proton acceptor; /evidence=ECO:0000250|UniProtKB:Q9LY46
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity
DNA Binding
EC Number 2.3.1.-
Enzyme Function FUNCTION: Xylan acetyltransferase required for 2-O- and 3-O-monoacetylation of xylosyl residues in xylan (PubMed:29569182, PubMed:27864442). Catalyzes the 2-O-acetylation of xylan, followed by nonenzymatic acetyl migration to the O-3 position, resulting in products that are monoacetylated at both O-2 and O-3 positions (By similarity). {ECO:0000250|UniProtKB:Q9LY46, ECO:0000269|PubMed:27864442, ECO:0000269|PubMed:29569182}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (3); Chain (1); Disulfide bond (4); Glycosylation (9); Motif (2); Topological domain (2); Transmembrane (1)
Keywords Disulfide bond;Glycoprotein;Golgi apparatus;Membrane;Plant defense;Reference proteome;Signal-anchor;Transferase;Transmembrane;Transmembrane helix
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Golgi apparatus membrane {ECO:0000250|UniProtKB:Q2QYU2}; Single-pass type II membrane protein {ECO:0000255}.
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif MOTIF 227..229; /note=GDS motif; /evidence=ECO:0000305|PubMed:29569182; MOTIF 479..482; /note=DXXH motif; /evidence=ECO:0000305|PubMed:29569182
Gene Encoded By
Mass 57,664
Kinetics BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=1.18 mM for xylohexaose {ECO:0000269|PubMed:29569182}; Vmax=51.6 pmol/min/mg enzyme with xylohexaose as substrate {ECO:0000269|PubMed:29569182};
Metal Binding
Rhea ID
Cross Reference Brenda