| IED ID | IndEnz0010001814 |
| Enzyme Type ID | esterase001814 |
| Protein Name |
Xyloglucan O-acetyltransferase 4 PtrXGOAT4 EC 2.3.1.- |
| Gene Name | XGOAT4 POPTR_017G073700 |
| Organism | Populus trichocarpa (Western balsam poplar) (Populus balsamifera subsp. trichocarpa) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids fabids Malpighiales Salicaceae Saliceae Populus (poplars) Populus trichocarpa (Western balsam poplar) (Populus balsamifera subsp. trichocarpa) |
| Enzyme Sequence | MTMHEKMKLPSCSCSAFKCGKKDRWLNMERPIPFLLIGLTTILSVFILYTLNPLKFVIEHNIDQKLLLIKPHKEEDKCDLFNGNWVPDFEGSIYTNSSCATIPTSKNCFRNGRKDQDFLNWRWKPERCDLPRFDATAYLDIVRGKTLAFIGDSVARNHIESLLCLLSQKEVPVDAYLDSEDRNRIWHFPVHNFTLKMLWTKFLVHGEERVINGSSSGIFDLYLDKVDENWARDLHSLDYVVISDAHWFFRQVYLHRGSNVVACVYCNEANVTDRGVAFALRMAFRAAFSQINHCNKCKGIVTLLRTFSPSHFENGFWNTGGSCNRTSPYNDQKINFGAYEWEIRSMQVEEIERAEKRGKKGKSFGVLDVTMAMLMRPDGHPGAFWGNQWMKGYNDCVHWCLPGPIDVWNDLLLAVLRRLD |
| Enzyme Length | 420 |
| Uniprot Accession Number | A0A2K1X4I9 |
| Absorption | |
| Active Site | ACT_SITE 153; /note=Nucleophile; /evidence=ECO:0000250|UniProtKB:Q9LY46; ACT_SITE 395; /note=Proton donor; /evidence=ECO:0000250|UniProtKB:Q9LY46; ACT_SITE 398; /note=Proton acceptor; /evidence=ECO:0000250|UniProtKB:Q9LY46 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 2.3.1.- |
| Enzyme Function | FUNCTION: Xyloglucan acetyltransferase that catalyzes the acetylation of fucosylated Gal residues on xyloglucan side chains (PubMed:30083810). Predominantly catalyze 6-O-monoacetylation of Gal residues in the Fuc-Gal-Xyl trisaccharide side chains of xyloglucan oligomers (PubMed:30083810). {ECO:0000269|PubMed:30083810}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Chain (1); Disulfide bond (4); Glycosylation (5); Motif (2); Topological domain (2); Transmembrane (1) |
| Keywords | Disulfide bond;Glycoprotein;Golgi apparatus;Membrane;Reference proteome;Signal-anchor;Transferase;Transmembrane;Transmembrane helix |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Golgi apparatus membrane {ECO:0000305}; Single-pass type II membrane protein {ECO:0000305}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | MOTIF 151..153; /note=GDS motif; /evidence=ECO:0000305|PubMed:30083810; MOTIF 395..398; /note=DXXH motif; /evidence=ECO:0000305|PubMed:30083810 |
| Gene Encoded By | |
| Mass | 48,615 |
| Kinetics | BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=4.15 mM for xyloglucan oligomer {ECO:0000269|PubMed:30083810}; Vmax=106.4 pmol/min/mg enzyme with xyloglucan oligomer as substrate {ECO:0000269|PubMed:30083810}; |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |