Detail Information for IndEnz0010001814
IED ID IndEnz0010001814
Enzyme Type ID esterase001814
Protein Name Xyloglucan O-acetyltransferase 4
PtrXGOAT4
EC 2.3.1.-
Gene Name XGOAT4 POPTR_017G073700
Organism Populus trichocarpa (Western balsam poplar) (Populus balsamifera subsp. trichocarpa)
Taxonomic Lineage cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids fabids Malpighiales Salicaceae Saliceae Populus (poplars) Populus trichocarpa (Western balsam poplar) (Populus balsamifera subsp. trichocarpa)
Enzyme Sequence MTMHEKMKLPSCSCSAFKCGKKDRWLNMERPIPFLLIGLTTILSVFILYTLNPLKFVIEHNIDQKLLLIKPHKEEDKCDLFNGNWVPDFEGSIYTNSSCATIPTSKNCFRNGRKDQDFLNWRWKPERCDLPRFDATAYLDIVRGKTLAFIGDSVARNHIESLLCLLSQKEVPVDAYLDSEDRNRIWHFPVHNFTLKMLWTKFLVHGEERVINGSSSGIFDLYLDKVDENWARDLHSLDYVVISDAHWFFRQVYLHRGSNVVACVYCNEANVTDRGVAFALRMAFRAAFSQINHCNKCKGIVTLLRTFSPSHFENGFWNTGGSCNRTSPYNDQKINFGAYEWEIRSMQVEEIERAEKRGKKGKSFGVLDVTMAMLMRPDGHPGAFWGNQWMKGYNDCVHWCLPGPIDVWNDLLLAVLRRLD
Enzyme Length 420
Uniprot Accession Number A0A2K1X4I9
Absorption
Active Site ACT_SITE 153; /note=Nucleophile; /evidence=ECO:0000250|UniProtKB:Q9LY46; ACT_SITE 395; /note=Proton donor; /evidence=ECO:0000250|UniProtKB:Q9LY46; ACT_SITE 398; /note=Proton acceptor; /evidence=ECO:0000250|UniProtKB:Q9LY46
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity
DNA Binding
EC Number 2.3.1.-
Enzyme Function FUNCTION: Xyloglucan acetyltransferase that catalyzes the acetylation of fucosylated Gal residues on xyloglucan side chains (PubMed:30083810). Predominantly catalyze 6-O-monoacetylation of Gal residues in the Fuc-Gal-Xyl trisaccharide side chains of xyloglucan oligomers (PubMed:30083810). {ECO:0000269|PubMed:30083810}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (3); Chain (1); Disulfide bond (4); Glycosylation (5); Motif (2); Topological domain (2); Transmembrane (1)
Keywords Disulfide bond;Glycoprotein;Golgi apparatus;Membrane;Reference proteome;Signal-anchor;Transferase;Transmembrane;Transmembrane helix
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Golgi apparatus membrane {ECO:0000305}; Single-pass type II membrane protein {ECO:0000305}.
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif MOTIF 151..153; /note=GDS motif; /evidence=ECO:0000305|PubMed:30083810; MOTIF 395..398; /note=DXXH motif; /evidence=ECO:0000305|PubMed:30083810
Gene Encoded By
Mass 48,615
Kinetics BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=4.15 mM for xyloglucan oligomer {ECO:0000269|PubMed:30083810}; Vmax=106.4 pmol/min/mg enzyme with xyloglucan oligomer as substrate {ECO:0000269|PubMed:30083810};
Metal Binding
Rhea ID
Cross Reference Brenda