| IED ID | IndEnz0010001816 |
| Enzyme Type ID | esterase001816 |
| Protein Name |
Xylan O-acetyltransferase 3 EC 2.3.1.- Protein trichome birefringence-like 9 OsTBL9 |
| Gene Name | XOAT3 TBL9 Os12g0104700 LOC_Os12g01380 OsJ_34909 |
| Organism | Oryza sativa subsp. japonica (Rice) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae Liliopsida Petrosaviidae commelinids Poales Poaceae BOP clade Oryzoideae Oryzeae Oryzinae Oryza Oryza sativa (Rice) Oryza sativa subsp. japonica (Rice) |
| Enzyme Sequence | MSKPQQQSPPSTTTTSPPPPPPSTPPPASSSRSLLSALRRSPVTTLVAAFFLLALFMYGEDVRTLAELSIDDYLYPDADFYNVSALPPLLLPPPTCDLSRGRWVFDNTSLPAYREKECTFLTKQVSCLANGRPDDLWQYWRWQPNNCSLPTFDARRFMEKMRGKRMMFVGDSLNRNQWESLVCLVQPILSKGRKKIVKRGSFNIFYAKEYRATLEFYWAPFLVESNSDNPNFHHIDQRIISPERIESHANNWKDVDYLIFNTYIWWMNNEDIKVRRPNSTSWSDHDEVPRIETYGRVFKTWSTWLEQNVDPARTSVFFMTISPLHNSPAQWGNPNGIKCVKETLPVLNYTKPLDLNHDMRMYDLVAKVAKNMKNVPVSLIDITRMSDYRKDAHTSLYSIRQGKLLTPEQKADPQKYADCIHWCLPGVPDVWNQILYTRILSKSSPPSTHPSLPPQ |
| Enzyme Length | 455 |
| Uniprot Accession Number | Q2QYW3 |
| Absorption | |
| Active Site | ACT_SITE 172; /note=Nucleophile; /evidence=ECO:0000250|UniProtKB:Q9LY46; ACT_SITE 418; /note=Proton donor; /evidence=ECO:0000250|UniProtKB:Q9LY46; ACT_SITE 421; /note=Proton acceptor; /evidence=ECO:0000250|UniProtKB:Q9LY46 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 2.3.1.- |
| Enzyme Function | FUNCTION: Xylan acetyltransferase required for 2-O- and 3-O-monoacetylation of xylosyl residues in xylan (PubMed:29569182). Catalyzes the 2-O-acetylation of xylan, followed by nonenzymatic acetyl migration to the O-3 position, resulting in products that are monoacetylated at both O-2 and O-3 positions (By similarity). {ECO:0000250|UniProtKB:Q9LY46, ECO:0000269|PubMed:29569182}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Chain (1); Disulfide bond (4); Glycosylation (5); Motif (2); Sequence conflict (1); Topological domain (2); Transmembrane (1) |
| Keywords | Disulfide bond;Glycoprotein;Golgi apparatus;Membrane;Reference proteome;Signal-anchor;Transferase;Transmembrane;Transmembrane helix |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Golgi apparatus membrane {ECO:0000250|UniProtKB:Q2QYU2}; Single-pass type II membrane protein {ECO:0000255}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | MOTIF 170..172; /note=GDS motif; /evidence=ECO:0000305|PubMed:29569182; MOTIF 418..421; /note=DXXH motif; /evidence=ECO:0000305|PubMed:29569182 |
| Gene Encoded By | |
| Mass | 52,585 |
| Kinetics | BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=1.82 mM for xylohexaose {ECO:0000269|PubMed:29569182}; Vmax=94 pmol/min/mg enzyme with xylohexaose as substrate {ECO:0000269|PubMed:29569182}; |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |