| IED ID | IndEnz0010001829 |
| Enzyme Type ID | esterase001829 |
| Protein Name |
Sterol esterase 1 EC 3.1.1.13 Steryl ester hydrolase 1 |
| Gene Name | YEH1 YLL012W L1329 |
| Organism | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Saccharomycotina (true yeasts) Saccharomycetes Saccharomycetales Saccharomycetaceae Saccharomyces Saccharomyces cerevisiae (Baker's yeast) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
| Enzyme Sequence | MGVSAVLKRARNLLATFIVCCFMAVVLVLALAHHFINEHRDTRSSSTQIEVDDESKRNVHHDHVLTRTNAYATPYLDLEHDKKNGIVYDHTRTVVRKKNHEVGSSSLHKNLFHKFLTKLIFRFIEKEKVTEGVTQGKFNNSSNEIANHEPVFEKIPVQCDNPLQNLILSEDLTLVADLNYYFNQYNIQIEEFRLETEDGFVIDLWHLIPKYRTTDSDKKKRPPILMLHGLLQSSGSFASNGRKSLAYFLYQSGYDIWLGNNRCGFRPEWNEAKVPTLASRWDWDLREMVKYDLTLLIDTVLAKTQFEKLTLISHSQGTTQGFMGLVNEDKFFPPGSGSKESFFTSKIANYIALAPAVYPGPLLNEKLFVKLMTKEIENPWFFGETSFFEIMMIVRNLCVGESLFSFVCYTIFNYLFDWNDTLWDTALRDRHFLFSPVHVSVKLMQWWLSPDPNKVSFKFGSHKMFPDNVKWFSDASKAPNIYLFVPKQDRLVDGERLINHFVNVESNVNYKIWYIDEYAHIDVLWAHDVIERIGKPILQNLNNYYSKKPSSAFESDCSDTEVETELEMVAEKA |
| Enzyme Length | 573 |
| Uniprot Accession Number | Q07804 |
| Absorption | |
| Active Site | ACT_SITE 315; /note=Nucleophile; /evidence=ECO:0000250; ACT_SITE 489; /note=Charge relay system; /evidence=ECO:0000250; ACT_SITE 520; /note=Charge relay system; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=a sterol ester + H2O = a fatty acid + a sterol + H(+); Xref=Rhea:RHEA:10100, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15889, ChEBI:CHEBI:28868, ChEBI:CHEBI:35915; EC=3.1.1.13; Evidence={ECO:0000269|PubMed:15713625}; |
| DNA Binding | |
| EC Number | 3.1.1.13 |
| Enzyme Function | FUNCTION: Mediates the hydrolysis of steryl esters, thereby playing a central role in lipid metabolism. Under heme-deficient conditions, it constitutes the major steryl ester hydrolase, suggesting that it plays a central role in mobilization of steryl esters under anaerobic conditions. {ECO:0000269|PubMed:15713625, ECO:0000269|PubMed:16835446}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Chain (1); Intramembrane (1); Topological domain (2) |
| Keywords | Hydrolase;Lipid degradation;Lipid droplet;Lipid metabolism;Membrane;Reference proteome |
| Interact With | |
| Induction | INDUCTION: Under heme-deficiency conditions. Heme-deficient induction requires ROX3. {ECO:0000269|PubMed:16835446}. |
| Subcellular Location | SUBCELLULAR LOCATION: Lipid droplet. Membrane; Peripheral membrane protein. |
| Modified Residue | |
| Post Translational Modification | PTM: Not N-glycosylated. |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 11283351; 16246075; 16429126; 16919863; 17434796; 18258205; 18269180; 18467557; 19111628; 19536198; 19696439; 21693588; 22194828; 22345606; 23139841; 23275493; 24597968; 25720564; 25860149; 25894691; 26004510; 26162625; 26456335; 26636650; 28772; 28866104; 8810043; |
| Motif | |
| Gene Encoded By | |
| Mass | 66,508 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:10100 |
| Cross Reference Brenda | 3.1.1.13; |