| IED ID | IndEnz0010001837 |
| Enzyme Type ID | esterase001837 |
| Protein Name |
Carboxymethylenebutenolidase 1 EC 3.1.1.45 Carboxymethylenebutenolidase I Dienelactone hydrolase I DLH I |
| Gene Name | tfdEI tfdE Reut_D6464 |
| Organism | Cupriavidus pinatubonensis (strain JMP 134 / LMG 1197) (Cupriavidus necator (strain JMP 134)) |
| Taxonomic Lineage | cellular organisms Bacteria Proteobacteria Betaproteobacteria Burkholderiales Burkholderiaceae Cupriavidus Cupriavidus pinatubonensis Cupriavidus pinatubonensis (strain JMP 134 / LMG 1197) (Cupriavidus necator (strain JMP 134)) |
| Enzyme Sequence | MLSDGVEITSRSGGRFGAYLGKPTTDSAPIVVIAQEIFGITPFIRETVEWLVGAGFGCVCPDLYWRQAPNIELDANVPSEREQALALFRDFDMEAGVNDLSCAIEYARALPFSNGRVAVVGYCLGGALAFDVAARSLADCSIGYYGVGLEKKVSLVPAITRPAMFHMGTKDHYVTEEARSILEEHFGRNKNLSLHWYPVGHSFARSSSPNFDQAATTVANARTLELLAMLKDPS |
| Enzyme Length | 234 |
| Uniprot Accession Number | P27136 |
| Absorption | |
| Active Site | ACT_SITE 123; /evidence=ECO:0000250; ACT_SITE 171; /evidence=ECO:0000250; ACT_SITE 201; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=2-(5-oxo-2,5-dihydrofuran-2-ylidene)acetate + H2O = 4-oxohex-2-enedioate + H(+); Xref=Rhea:RHEA:12372, ChEBI:CHEBI:12040, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:57263; EC=3.1.1.45; |
| DNA Binding | |
| EC Number | 3.1.1.45 |
| Enzyme Function | FUNCTION: Ring cleavage of cyclic ester dienelactone to produce maleylacetate. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | PATHWAY: Aromatic compound metabolism; 3-chlorocatechol degradation. |
| nucleotide Binding | |
| Features | Active site (3); Chain (1) |
| Keywords | Aromatic hydrocarbons catabolism;Hydrolase;Plasmid;Serine esterase |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | Plasmid pJP4; Plasmid pPJ4 |
| Mass | 25,428 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:12372 |
| Cross Reference Brenda |