| IED ID | IndEnz0010001849 |
| Enzyme Type ID | esterase001849 |
| Protein Name |
Xylan O-acetyltransferase 1 EC 2.3.1.- Protein ESKIMO 1 Protein trichome birefringence-like 29 |
| Gene Name | ESK1 TBL29 XOAT1 At3g55990 F27K19.170 |
| Organism | Arabidopsis thaliana (Mouse-ear cress) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress) |
| Enzyme Sequence | MQPWRRKFPLFETGVTMKQRKNSNLSIFVVVFSVFLFGIFMYNEDVKSIAEFPFSTSKPHDVHDEATPITEITTLPVQESIKNSDPIQESIKNADSVQDSVKDVAEPVQEEVSKTEEVKKIELFAATEDEEDVELPPEECDLFTGEWVFDNETHPLYKEDQCEFLTAQVTCMRNGRRDSLYQNWRWQPRDCSLPKFKAKLLLEKLRNKRMMFVGDSLNRNQWESMVCLVQSVVPPGRKSLNKTGSLSVFRVEDYNATVEFYWAPFLVESNSDDPNMHSILNRIIMPESIEKHGVNWKGVDFLVFNTYIWWMNTFAMKVLRGSFDKGDTEYEEIERPVAYRRVMRTWGDWVERNIDPLRTTVFFASMSPLHIKSLDWENPDGIKCALETTPILNMSMPFSVGTDYRLFSVAENVTHSLNVPVYFLNITKLSEYRKDAHTSVHTIRQGKMLTPEQQADPNTYADCIHWCLPGLPDTWNEFLYTRIISRS |
| Enzyme Length | 487 |
| Uniprot Accession Number | Q9LY46 |
| Absorption | |
| Active Site | ACT_SITE 216; /note=Nucleophile; /evidence=ECO:0000269|PubMed:32354790; ACT_SITE 462; /note=Proton donor; /evidence=ECO:0000269|PubMed:32354790; ACT_SITE 465; /note=Proton acceptor; /evidence=ECO:0000269|PubMed:32354790 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 2.3.1.- |
| Enzyme Function | FUNCTION: Xylan acetyltransferase required for 2-O- and 3-O-monoacetylation of xylosyl residues in xylan (PubMed:23659919, PubMed:30083810, PubMed:32354790). Catalyzes the 2-O-acetylation of xylan, followed by nonenzymatic acetyl migration to the O-3 position, resulting in products that are monoacetylated at both O-2 and O-3 positions (PubMed:32354790). Is necessary for the formation of the functional xylem, which is required for water transport to aerial tissues (PubMed:21408051, PubMed:23340742). Acts as negative regulator of cold acclimation (PubMed:17316173). Involved in water economy as well as salt tolerance (PubMed:19061521, PubMed:19054354). Regulated at the transcriptional level by NAC012/SND1 (Probable). {ECO:0000269|PubMed:17316173, ECO:0000269|PubMed:19054354, ECO:0000269|PubMed:19061521, ECO:0000269|PubMed:21408051, ECO:0000269|PubMed:23340742, ECO:0000269|PubMed:23659919, ECO:0000269|PubMed:30083810, ECO:0000269|PubMed:32354790, ECO:0000305}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Beta strand (17); Chain (1); Disulfide bond (4); Glycosylation (6); Helix (15); Modified residue (1); Motif (2); Mutagenesis (10); Sequence conflict (1); Topological domain (2); Transmembrane (1); Turn (7) |
| Keywords | 3D-structure;Acetylation;Disulfide bond;Glycoprotein;Golgi apparatus;Membrane;Reference proteome;Signal-anchor;Transferase;Transmembrane;Transmembrane helix |
| Interact With | |
| Induction | INDUCTION: Not induced during cold acclimation. {ECO:0000269|PubMed:17316173}. |
| Subcellular Location | SUBCELLULAR LOCATION: Golgi apparatus membrane {ECO:0000305}; Single-pass type II membrane protein {ECO:0000305}. |
| Modified Residue | MOD_RES 216; /note=O-acetylserine; /evidence=ECO:0000269|PubMed:32354790 |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | X-ray crystallography (1) |
| Cross Reference PDB | 6CCI; |
| Mapped Pubmed ID | 16581939; 18467456; 19026725; 23517122; 24518588; 24521940; 25141999; 25184213; 25888614; 26556650; 26745802; 26795157; 27247031; 28792629; 28993612; 29059346; 31245725; 31245785; 32732351; 9636231; |
| Motif | MOTIF 214..216; /note=GDS motif; /evidence=ECO:0000305|PubMed:30083810; MOTIF 462..465; /note=DXXH motif; /evidence=ECO:0000305|PubMed:30083810 |
| Gene Encoded By | |
| Mass | 56,695 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |