| IED ID | IndEnz0010001862 |
| Enzyme Type ID | esterase001862 |
| Protein Name |
Xylan O-acetyltransferase 6 EC 2.3.1.- Protein trichome birefringence-like 15 OsTBL15 |
| Gene Name | XOAT6 TBL15 Os03g0291800 LOC_Os03g18140 |
| Organism | Oryza sativa subsp. japonica (Rice) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae Liliopsida Petrosaviidae commelinids Poales Poaceae BOP clade Oryzoideae Oryzeae Oryzinae Oryza Oryza sativa (Rice) Oryza sativa subsp. japonica (Rice) |
| Enzyme Sequence | MQQRRKSVFASAPFAMKQAALGAGVAARRNGAPLSLAAVVFALFVFATFLYNEDIKSIADFPFGAGALRAKSPDLHVLQETVGAAHLAAGSIAKRGEEVIVRVLDAPASTAMAAAAGSSSNNSTIEVAKANANANANAADAGVKVDEGQERERDVTLPSVKEGGADEARRREDEEAAEKESSAKAAAATAALRTVVSVPDTCDLYRGNWVYDEVNAPVYKESQCEFLTEQVTCMRNGRRDDSYQKWRWQPTDCDLPRFDARLLLERLRNKRLMFVGDSLNRNQWESMVCLVQSVIPKGKKTLTKFVNGGNSNIFYAHEYNATVEFYWAPFLVESNSDNPQVHSVPDRVIQWHSIAKHAHNWLGVDYLIFNTYIWWLNTLDMKVLKGSFDQGATEYVEVDRPVAYKEVLKTWAKWVDRNIDPNRTTVFFMSMSPNHITPEAWGNYGGIKCAMETLPITNRTTSLDVGTDWRLYAGAQEVLQTFRRVPVHLVDITALSELRKDAHTSVHTLRQGKLLTPEQQSDPKTYADCIHWCLPGLPDTWNQFLYARIASAPWSSDQ |
| Enzyme Length | 558 |
| Uniprot Accession Number | Q10MX2 |
| Absorption | |
| Active Site | ACT_SITE 278; /note=Nucleophile; /evidence=ECO:0000250|UniProtKB:Q9LY46; ACT_SITE 528; /note=Proton donor; /evidence=ECO:0000250|UniProtKB:Q9LY46; ACT_SITE 531; /note=Proton acceptor; /evidence=ECO:0000250|UniProtKB:Q9LY46 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 2.3.1.- |
| Enzyme Function | FUNCTION: Xylan acetyltransferase required for 2-O- and 3-O-monoacetylation of xylosyl residues in xylan (PubMed:29569182). Catalyzes the 2-O-acetylation of xylan, followed by nonenzymatic acetyl migration to the O-3 position, resulting in products that are monoacetylated at both O-2 and O-3 positions (By similarity). {ECO:0000250|UniProtKB:Q9LY46, ECO:0000269|PubMed:29569182}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Chain (1); Disulfide bond (4); Erroneous initiation (1); Glycosylation (4); Motif (2); Topological domain (2); Transmembrane (1) |
| Keywords | Disulfide bond;Glycoprotein;Golgi apparatus;Membrane;Reference proteome;Signal-anchor;Transferase;Transmembrane;Transmembrane helix |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Golgi apparatus membrane {ECO:0000250|UniProtKB:Q2QYU2}; Single-pass type II membrane protein {ECO:0000255}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | MOTIF 276..278; /note=GDS motif; /evidence=ECO:0000305|PubMed:29569182; MOTIF 528..531; /note=DXXH motif; /evidence=ECO:0000305|PubMed:29569182 |
| Gene Encoded By | |
| Mass | 62,216 |
| Kinetics | BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=35 uM for xylohexaose {ECO:0000269|PubMed:29569182}; Vmax=14.4 pmol/min/mg enzyme with xylohexaose as substrate {ECO:0000269|PubMed:29569182}; |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |