| IED ID | IndEnz0010001927 |
| Enzyme Type ID | esterase001927 |
| Protein Name |
Xylan O-acetyltransferase 7 EC 2.3.1.- Protein trichome birefringence-like 14 OsTBL14 |
| Gene Name | XOAT7 TBL14 Os07g0693600 LOC_Os07g49280 OsJ_25693 |
| Organism | Oryza sativa subsp. japonica (Rice) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae Liliopsida Petrosaviidae commelinids Poales Poaceae BOP clade Oryzoideae Oryzeae Oryzinae Oryza Oryza sativa (Rice) Oryza sativa subsp. japonica (Rice) |
| Enzyme Sequence | MKKKKNGMGAAADRGRLLALAHHDKLNPTKPSEAQRRFKPSILLLLGSSLPRLVPPLPSSFLPVVIKQTEFHQRWLVGDLNPPPPPCHLLPIQGQGQMQMQQRRKPPPAAAPVAAKQPSPRRTPGPLSFAGALLSLLVVATFLYINDHGNMMPPHASPDPDLRLLQEAAHQKVNSILLSRHAPAPPPRTNTNTSSSDQHLRLINIPMSSDLDLELGGNSTSSSGVEIQFEQQQQQEEKNLRGCELYKGRWVYDAAGREAPLYRESECGFLTEQVTCMRNGRRDDSYQRWRWQPEGCDLPSFDARALLERLRNKRMMFVGDSLNRNQWESMVCLVQSAIPYGQKTLTKFVNNGSLNVFRAHEYNATVEFYWAPFLVQSNSDDPQVHSVRDRVIAWRSIAKHAANWKGVHYLVFNTYIWWLNNFQIKVLKSRGAPFAGSGGWSSRYALVDRAIAYREVLKTWAKWVDRRIDPNKTHVFFMAMSPNHFMPEAWGGSAGAVKCAMETQPIVNRTSGGLDIGTDWRLHGVARGVLRSMRRVGVRFVDITALSELRKDAHTSVHTLRQGKLLTPEQQADPRTYADCIHWCLPGLPDTWNHFLYAHIVAHAA |
| Enzyme Length | 605 |
| Uniprot Accession Number | Q0D3C8 |
| Absorption | |
| Active Site | ACT_SITE 321; /note=Nucleophile; /evidence=ECO:0000250|UniProtKB:Q9LY46; ACT_SITE 579; /note=Proton donor; /evidence=ECO:0000250|UniProtKB:Q9LY46; ACT_SITE 582; /note=Proton acceptor; /evidence=ECO:0000250|UniProtKB:Q9LY46 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 2.3.1.- |
| Enzyme Function | FUNCTION: Xylan acetyltransferase required for 2-O- and 3-O-monoacetylation of xylosyl residues in xylan (PubMed:29569182). Catalyzes the 2-O-acetylation of xylan, followed by nonenzymatic acetyl migration to the O-3 position, resulting in products that are monoacetylated at both O-2 and O-3 positions (By similarity). {ECO:0000250|UniProtKB:Q9LY46, ECO:0000269|PubMed:29569182}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Chain (1); Disulfide bond (4); Glycosylation (6); Motif (2); Topological domain (2); Transmembrane (1) |
| Keywords | Disulfide bond;Glycoprotein;Golgi apparatus;Membrane;Reference proteome;Signal-anchor;Transferase;Transmembrane;Transmembrane helix |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Golgi apparatus membrane {ECO:0000250|UniProtKB:Q2QYU2}; Single-pass type II membrane protein {ECO:0000255}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | MOTIF 319..321; /note=GDS motif; /evidence=ECO:0000305|PubMed:29569182; MOTIF 579..582; /note=DXXH motif; /evidence=ECO:0000305|PubMed:29569182 |
| Gene Encoded By | |
| Mass | 68,270 |
| Kinetics | BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=60 uM for xylohexaose {ECO:0000269|PubMed:29569182}; Vmax=37.7 pmol/min/mg enzyme with xylohexaose as substrate {ECO:0000269|PubMed:29569182}; |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |