| IED ID | IndEnz0011000003 |
| Enzyme Type ID | glucanase000003 |
| Protein Name |
Glucan endo-1,3-beta-glucosidase 10 EC 3.2.1.39 1- 3 -beta-glucan endohydrolase 10 1- 3 -beta-glucanase 10 Beta-1,3-endoglucanase 10 Beta-1,3-glucanase 10 Putative plasmodesmal associated protein AtBG_ppap |
| Gene Name | At5g42100 MJC20.21 |
| Organism | Arabidopsis thaliana (Mouse-ear cress) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress) |
| Enzyme Sequence | MASSSLQSLFSLFCLALFSLPLIVSSIGINYGQVANNLPPPKNVIPLLKSVGATKVKLYDADPQALRAFAGSGFELTVALGNEYLAQMSDPIKAQGWVKENVQAYLPNTKIVAIVVGNEVLTSNQSALTAALFPAMQSIHGALVDCGLNKQIFVTTAHSLAILDVSYPPSATSFRRDLLGSLTPILDFHVKTGSPILINAYPFFAYEENPKHVSLDFVLFQPNQGFTDPGSNFHYDNMLFAQVDAVYHALDAVGISYKKVPIVVSETGWPSNGDPQEVGATCDNARKYNGNLIKMMMSKKMRTPIRPECDLTIFVFALFNENMKPGPTSERNYGLFNPDGTPVYSLGIKTSSTHSSGSGSSNSTGGSSSGGGGNTGGSSSGGGIYQPVTGNPSPDYMSISSAGGKGRFVECVLFFFLLCIIKLRL |
| Enzyme Length | 425 |
| Uniprot Accession Number | Q9FHX5 |
| Absorption | |
| Active Site | ACT_SITE 119; /note=Proton donor; /evidence=ECO:0000250|UniProtKB:O22317; ACT_SITE 266; /note=Nucleophile; /evidence=ECO:0000250|UniProtKB:O22317 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans.; EC=3.2.1.39; |
| DNA Binding | |
| EC Number | 3.2.1.39 |
| Enzyme Function | FUNCTION: Plasmodesmal-associated membrane beta-1,3-glucanase involved in plasmodesmal callose degradation and functions in the gating of plasmodesmata. {ECO:0000269|PubMed:17270015, ECO:0000269|PubMed:23656331}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Alternative sequence (1); Chain (1); Glycosylation (2); Lipidation (1); Propeptide (1); Region (1); Signal peptide (1) |
| Keywords | Alternative splicing;Cell junction;Cell membrane;Cell wall biogenesis/degradation;GPI-anchor;Glycoprotein;Glycosidase;Hydrolase;Lipoprotein;Membrane;Plant defense;Reference proteome;Signal |
| Interact With | |
| Induction | INDUCTION: By infection with the cucumber mosaic virus (CMV). {ECO:0000269|PubMed:23656331}. |
| Subcellular Location | SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:17270015}; Lipid-anchor, GPI-anchor {ECO:0000255}; Extracellular side {ECO:0000255}. Cell junction, plasmodesma {ECO:0000269|PubMed:17270015}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..26; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 12068095; 12805588; 14517339; 15517348; 16287169; 17317660; 19704615; 21282527; 27559035; 30466394; |
| Motif | |
| Gene Encoded By | |
| Mass | 45,358 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |