| IED ID | IndEnz0011000019 |
| Enzyme Type ID | glucanase000019 |
| Protein Name |
Glucan 1,3-beta-glucosidase EC 3.2.1.58 Exo-1,3-beta-glucanase GP29 Soluble cell wall protein 9 |
| Gene Name | BGL2 SCW9 YGR282C |
| Organism | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Saccharomycotina (true yeasts) Saccharomycetes Saccharomycetales Saccharomycetaceae Saccharomyces Saccharomyces cerevisiae (Baker's yeast) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
| Enzyme Sequence | MRFSTTLATAATALFFTASQVSAIGELAFNLGVKNNDGTCKSTSDYETELQALKSYTSTVKVYAASDCNTLQNLGPAAEAEGFTIFVGVWPTDDSHYAAEKAALQTYLPKIKESTVAGFLVGSEALYRNDLTASQLSDKINDVRSVVADISDSDGKSYSGKQVGTVDSWNVLVAGYNSAVIEASDFVMANAFSYWQGQTMQNASYSFFDDIMQALQVIQSTKGSTDITFWVGETGWPTDGTNFESSYPSVDNAKQFWKEGICSMRAWGVNVIVFEAFDEDWKPNTSGTSDVEKHWGVFTSSDNLKYSLDCDFS |
| Enzyme Length | 313 |
| Uniprot Accession Number | P15703 |
| Absorption | |
| Active Site | ACT_SITE 124; /note=Proton donor; /evidence=ECO:0000250|UniProtKB:O22317; ACT_SITE 233; /note=Nucleophile; /evidence=ECO:0000250|UniProtKB:O22317 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Successive hydrolysis of beta-D-glucose units from the non-reducing ends of (1->3)-beta-D-glucans, releasing alpha-glucose.; EC=3.2.1.58; |
| DNA Binding | |
| EC Number | 3.2.1.58 |
| Enzyme Function | FUNCTION: Glucanases possibly play a role in cell expansion during growth, in cell-cell fusion during mating, and in spore release during sporulation. This enzyme may be involved in beta-glucan degradation and also function biosynthetically as a transglycosylase. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Chain (1); Glycosylation (2); Signal peptide (1) |
| Keywords | Cell wall;Cell wall biogenesis/degradation;Chitin-binding;Direct protein sequencing;Glycoprotein;Glycosidase;Hydrolase;Reference proteome;Secreted;Signal |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted, cell wall {ECO:0000269|PubMed:9748433}. Note=Tightly bound to cell wall. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..23; /evidence="ECO:0000269|PubMed:8935650, ECO:0000269|PubMed:9748433" |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 11805826; 11805837; 12023081; 12209002; 14574112; 15256547; 15470095; 15470100; 15699485; 16429126; 16498706; 16760306; 16986442; 17651441; 17899389; 18680912; 19098439; 19129178; 19536198; 20489023; 20508643; 22261724; 22533807; 22553351; 23135325; 23208381; 23275495; 23419716; 23959528; 24391512; 25073740; 26270963; 27193513; 27693354; 28634239; 33167499; 7565410; 7851411; 7985414; 8152414; 8458852; |
| Motif | |
| Gene Encoded By | |
| Mass | 34,119 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |