| IED ID | IndEnz0011000020 |
| Enzyme Type ID | glucanase000020 |
| Protein Name |
Endo-1,6-beta-D-glucanase BGN16.3 EC 3.2.1.75 Beta-1,6-glucanase BGN16.3 Glucan endo-1,6-beta-glucosidase BGN16.3 |
| Gene Name | |
| Organism | Trichoderma harzianum (Hypocrea lixii) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta sordariomyceta Sordariomycetes Hypocreomycetidae Hypocreales Hypocreaceae Trichoderma Trichoderma harzianum (Hypocrea lixii) |
| Enzyme Sequence | MRYALIASMLGQAAISVAMPSEPAHSPRAAGAQAYASNQAGNYKLTSIAAPVQGNGSPGPSTWNLSIDDTSSGYKQKIVGFGAAVTDATVSAFNELSASTLSQLLDELMTGAGASFSLMRHTIGASDLSGDPAYTYDDNGGNADPGMTGFNLGDRGTAMATMLAQMKGLNSNLQIFGSPWSAPGWMKLNNAIDGNTNNNNLNDGYLTNNGAQYSAAFAQYFVKYIQAFESHGATINAITLQNEPLNSQAGYPTMYMFSYEQGDLIQNYVAPALKAAGLSTKIWAYDHNTDQPDFPEQVMGIAADDVSAVAWHCYATNLDWTVLTNFHNSYPNTDQYMTECWTPSTGAWNQAASFTMGPLQNWARGVAAWTLGTTAQDGPHLSSGGCGTCTGLVTINNGQYTFQTAYYMMAQFSKFMPVGATVLSGTGSYTYSGSGGVQSVASLNPDGTRTVVIENTFGNDIYIHLSTSSGQEWSGNVPTNSVTTWVLPAV |
| Enzyme Length | 490 |
| Uniprot Accession Number | Q8J0I9 |
| Absorption | |
| Active Site | ACT_SITE 243; /note=Proton donor; /evidence=ECO:0000250|UniProtKB:P04062; ACT_SITE 339; /note=Nucleophile; /evidence=ECO:0000250|UniProtKB:P04062 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Random hydrolysis of (1->6)-linkages in (1->6)-beta-D-glucans.; EC=3.2.1.75; Evidence={ECO:0000269|PubMed:15982190}; |
| DNA Binding | |
| EC Number | 3.2.1.75 |
| Enzyme Function | FUNCTION: Has highest activity on the linear beta-1,6-glucan pustulan. Lower activity against yeast glucan and laminarin (beta-1,3-glucans with beta-1,6-branches). No activity on colloidal chitin, pachyman, starch, cellulose, nigeran, dextran or gentobiose. {ECO:0000269|PubMed:15982190}. |
| Temperature Dependency | BIOPHYSICOCHEMICAL PROPERTIES: Temperature dependence: Optimum temperature is 50 degrees Celsius. After 30 min incubation at 50 degrees Celsius in the absence of substrate 50% of activity is lost. {ECO:0000269|PubMed:15982190}; |
| PH Dependency | BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 5.0. At least 20% of maximum activity is retained between pH 4.0 and 7.0. {ECO:0000269|PubMed:15982190}; |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Chain (1); Signal peptide (1) |
| Keywords | Direct protein sequencing;Glycosidase;Hydrolase;Secreted;Signal |
| Interact With | |
| Induction | INDUCTION: By fungal cell walls. {ECO:0000269|PubMed:15982190}. |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted, extracellular space {ECO:0000269|PubMed:15982190}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..28; /evidence=ECO:0000269|PubMed:15982190 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 51,890 |
| Kinetics | BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: Vmax=390 umol/min/mg enzyme {ECO:0000269|PubMed:15982190}; |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |