| IED ID | IndEnz0011000024 |
| Enzyme Type ID | glucanase000024 |
| Protein Name |
Endo-1,3 4 -beta-glucanase xgeA EC 3.2.1.6 Mixed-linked glucanase xgeA |
| Gene Name | xgeA AN2385 |
| Organism | Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta Eurotiomycetes Eurotiomycetidae Eurotiales (green and blue molds) Aspergillaceae Aspergillus Aspergillus subgen. Nidulantes Emericella nidulans (Aspergillus nidulans) Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans) |
| Enzyme Sequence | MSSSLMRRVGSLAASAIIFPGIAHAASNYKLKESWEGEKILNHFHFFDNADPTNGFVTYVNQSYAESAGLVKTTDSGSLYLGVDYENVLTVDGPGRESVRIESNEYYDQGLYVVDIQHMPGSICGTWPAFWTVGPDWPTDGEIDIIEGVNKHDANKIVLHTSDTCDVGGGYKMTGDMTSSECGEASGTIGCVVQGKQGSSGDPFNEQGGGVYAMEWQEKYLKIWYFPRSSIPESLTAGTPDVSSFGTPMAHLQGSCNFKERFTHQKLILDTTFCGDWAGGVFGDSGCPVSDPSDPMLSCKNYVAENPAVYKNAYWELNSIKIYQLGGTAEVEGTQSAAAESTAAEATAAETTAAATQTANGGSIEEITTSTHSVTRTKTVSATHSTETAAVTETAAATTAAASVASEVDATNTQPVSKTKSTSYVTSTTTLCPVESSQAAATESVSRTKTTSYVTITTTLCPVESLQTANAVPSAKASTDAAAATTPAAEPHPSNAETPADSKSSADAVTAQATKTTIAVNTPNPATDSASSVPPDSIVYTAPEVTSSSSVPLFTIVSSSSQFVTVPTAAPSSFEPTDAVRDGADSYSTAASPTTPSNPVFTGVGSKVSISASVAIAAFVMLLLVN |
| Enzyme Length | 626 |
| Uniprot Accession Number | Q5BAP5 |
| Absorption | |
| Active Site | ACT_SITE 142; /note=Nucleophile; /evidence=ECO:0000250; ACT_SITE 147; /note=Proton donor; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Endohydrolysis of (1->3)- or (1->4)-linkages in beta-D-glucans when the glucose residue whose reducing group is involved in the linkage to be hydrolyzed is itself substituted at C-3.; EC=3.2.1.6; |
| DNA Binding | |
| EC Number | 3.2.1.6 |
| Enzyme Function | FUNCTION: Mixed-linked glucanase involved in the degradation of complex natural cellulosic substrates. Active on laminarin. lichenan, soluble carboxymethyl cellulose but not on pustulan. {ECO:0000269|PubMed:16844780}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Chain (1); Compositional bias (1); Domain (1); Glycosylation (1); Lipidation (1); Propeptide (1); Region (1); Signal peptide (1) |
| Keywords | Carbohydrate metabolism;Cell membrane;Cellulose degradation;GPI-anchor;Glycoprotein;Glycosidase;Hydrolase;Lipoprotein;Membrane;Polysaccharide degradation;Reference proteome;Signal |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Lipid-anchor, GPI-anchor {ECO:0000250}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..25; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 65,098 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |