| IED ID | IndEnz0011000025 |
| Enzyme Type ID | glucanase000025 |
| Protein Name |
Glucan endo-1,3-alpha-glucosidase agn1 EC 3.2.1.59 Endo-1,3-alpha-glucanase agn1 |
| Gene Name | agn1 SPAC14C4.09 |
| Organism | Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota Taphrinomycotina Schizosaccharomycetes Schizosaccharomycetales Schizosaccharomycetaceae Schizosaccharomyces Schizosaccharomyces pombe (Fission yeast) Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) |
| Enzyme Sequence | MKLVLFLVLLFSALINLTNADKMVVAHFIVGNTYPYTVSNWEEDIQDAIAVGIDGFALNMGSDAWQVERIEDAYDAAASVSSDFKLFISFDMSIISADADFIEGVVRRFADKPNQLYYDGKVFVSTFAGETDTFGYSDVSTGWDSAVKEPLASAGYPIYFVPSWTSLGQGALEESVADGFLSWNAWPTTDADMNDNDDIGYQNLANSLGKLYVAPVSPWFYTHLSYKNWAYKSDWLIIDRWNEMLSVQPDMIEVLTWNDYGESHYIGNIQGALPAGSEGYVDGFDHTAWRYLMSPYISAYKLGLSEPYINFESLFYWYRPTPKSATATADSLSYPSGGDYMEDEIFVLVYLLQSAEVTVTCGSTTQTFSGVPGVNQFTIPMETNASPSFTVARQGGTLASGTGPEIVDSLSIYNFNAYTGVLYF |
| Enzyme Length | 424 |
| Uniprot Accession Number | O13716 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Endohydrolysis of (1->3)-alpha-D-glucosidic linkages in isolichenin, pseudonigeran and nigeran.; EC=3.2.1.59; |
| DNA Binding | |
| EC Number | 3.2.1.59 |
| Enzyme Function | FUNCTION: Has a role in cell separation where it is required for the degradation of the cell wall material surrounding the septum (the septum edging) which must be hydrolyzed before full separation of the daughter cells can occur. Hydrolyzes 1,3-alpha-glucan predominantly into pentasaccharides. {ECO:0000269|PubMed:15194814}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Chain (1); Signal peptide (1) |
| Keywords | Cell cycle;Cell division;Cell wall;Cell wall biogenesis/degradation;Glycosidase;Hydrolase;Reference proteome;Secreted;Signal |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:15194814}. Secreted, cell wall {ECO:0000269|PubMed:15194814}. Note=Associates with the cell wall. |
| Modified Residue | |
| Post Translational Modification | PTM: Not glycosylated. |
| Signal Peptide | SIGNAL 1..20; /evidence=ECO:0000305 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 20473289; 23093943; 23697806; 26890608; 28410370; 34250083; |
| Motif | |
| Gene Encoded By | |
| Mass | 46,974 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |