Detail Information for IndEnz0011000037
IED ID IndEnz0011000037
Enzyme Type ID glucanase000037
Protein Name Glucan endo-1,3-beta-glucosidase
EC 3.2.1.39
Beta-1,3-glucanase
allergen Mus a 5
Gene Name BANGLUC
Organism Musa acuminata (Banana) (Musa cavendishii)
Taxonomic Lineage cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae Liliopsida Petrosaviidae commelinids Zingiberales Musaceae Musa Musa acuminata (Banana) (Musa cavendishii)
Enzyme Sequence MATKASLSIKGFALLVSVLVAVPTRVQSIGVCYGMLGNNLPPPSEVVSLYKSNNIARMRLYDPNQAALQALRNSNIQVLLDVPRSDVQSLASNPSAAGDWIRRNVVAYWPSVSFRYIAVGNELIPGSDLAQYILPAMRNIYNALSSAGLQNQIKVSTAVDTGVLGTSYPPSAGAFSSAAQAYLSPIVQFLASNGAPLLVNVYPYFSYTGNPGQISLPYALFTASGVVVQDGRFSYQNLFDAIVDAVFAALERVGGANVAVVVSESGWPSAGGGAEASTSNARTYNQNLIRHVGGGTPRRPGKEIEAYIFEMFNENQKAGGIEQNFGLFYPNKQPVYQISF
Enzyme Length 340
Uniprot Accession Number O22317
Absorption
Active Site ACT_SITE 122; /note=Proton donor; /evidence=ECO:0000305|PubMed:16421930; ACT_SITE 264; /note=Nucleophile; /evidence=ECO:0000305|PubMed:16421930
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans.; EC=3.2.1.39; Evidence={ECO:0000269|PubMed:10672030};
DNA Binding
EC Number 3.2.1.39
Enzyme Function FUNCTION: Possesses beta-1,3-endoglucanase activity in vitro (PubMed:10672030). May play a role in fruit pulp softening process (Probable). Can cleave beta-1,6-branched glucans in vitro (PubMed:16421930). {ECO:0000269|PubMed:10672030, ECO:0000269|PubMed:16421930, ECO:0000305|PubMed:10672030, ECO:0000305|PubMed:20467747}.
Temperature Dependency
PH Dependency BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 4.5. {ECO:0000269|PubMed:10672030};
Pathway
nucleotide Binding
Features Active site (2); Beta strand (13); Chain (1); Helix (17); Sequence conflict (6); Signal peptide (1); Turn (5)
Keywords 3D-structure;Allergen;Direct protein sequencing;Glycosidase;Hydrolase;Signal
Interact With
Induction INDUCTION: Induced by ethylene (PubMed:20467747). Down-regulated by exposure to constant white light (PubMed:20467747). {ECO:0000269|PubMed:20467747}.
Subcellular Location
Modified Residue
Post Translational Modification
Signal Peptide SIGNAL 1..28; /evidence=ECO:0000269|PubMed:22162266
Structure 3D X-ray crystallography (1)
Cross Reference PDB 2CYG;
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 36,321
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda