Detail Information for IndEnz0011000044
IED ID IndEnz0011000044
Enzyme Type ID glucanase000044
Protein Name Glucan endo-1,3-beta-D-glucosidase
EC 3.2.1.39
Major pollen allergen Ole e 9
allergen Ole e 9
Gene Name OLE9
Organism Olea europaea (Common olive)
Taxonomic Lineage cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae asterids lamiids Lamiales Oleaceae Oleeae Olea Olea europaea (Common olive)
Enzyme Sequence MAANVQTSSLLFLVFLLLQNFYSANSQSFLGVNYGQLSDNLPSLQATVNLLKSTTIQKVRLFGAEPAVIKAFANTGVEIVIGFDNGDIPTLASNPNVASQFVKSNVMSFYPASNIIAITVGNEVLTSGDQKLISQLLPAMQNVQNALNAASLGGKVKVSTVHAMAVLSQSYPPSSGVFNPGLGDTMKALLQFQSANDAPFMISPYPYFAYKNQPTPDTLAFCLFQPNAGQVDSGNGHKYTNMFDAQVDAVHSALNAMGFKDIEIVVAETGWPHGGDSNEVGPSLDNAKAYVGNLINHLKSKVGTPLMPGKSIDTYLFSLYDEDKKTGASSEKYFGLFKPDGSTTYDVGLLKNTQNPTTPATPTPTPKAAGSWCVPKPGVSDDQLTGNINYACGQGIDCGPIQPGGACFEPNTVKAHAAYVMNLYYQSAGRNSWNCDFSQTATLTNTNPSYGACNFPSGSN
Enzyme Length 460
Uniprot Accession Number Q94G86
Absorption
Active Site ACT_SITE 123; /note=Proton donor; /evidence=ECO:0000250|UniProtKB:O22317; ACT_SITE 268; /note=Nucleophile; /evidence=ECO:0000250|UniProtKB:O22317
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans.; EC=3.2.1.39; Evidence={ECO:0000269|PubMed:11373288};
DNA Binding
EC Number 3.2.1.39
Enzyme Function
Temperature Dependency
PH Dependency BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 4.5-6.0. {ECO:0000269|PubMed:11373288};
Pathway
nucleotide Binding
Features Active site (2); Beta strand (5); Chain (1); Disulfide bond (1); Glycosylation (2); Helix (3); Region (1); Signal peptide (1); Turn (1)
Keywords 3D-structure;Allergen;Direct protein sequencing;Disulfide bond;Glycoprotein;Glycosidase;Hydrolase;Secreted;Signal
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
Modified Residue
Post Translational Modification PTM: Glycosylated. {ECO:0000269|PubMed:11373288, ECO:0000269|PubMed:12392450}.; PTM: Contains two additional disulfide bonds, but it is unclear if they are between the pairs Cys-392-Cys-398 and Cys-407-Cys-453 (PudMed:18096638) or between the pairs Cys-392-Cys-453 and Cys-398-Cys-407 (PudMed:12392450).
Signal Peptide SIGNAL 1..26; /evidence=ECO:0000255
Structure 3D NMR spectroscopy (1)
Cross Reference PDB 2JON;
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 48,838
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda