IED ID | IndEnz0011000048 |
Enzyme Type ID | glucanase000048 |
Protein Name |
Exoglucanase A EC 3.2.1.91 1,4-beta-cellobiohydrolase A CBP95 Exocellobiohydrolase A |
Gene Name | cbhA Celf_1925 |
Organism | Cellulomonas fimi (strain ATCC 484 / DSM 20113 / JCM 1341 / NBRC 15513 / NCIMB 8980 / NCTC 7547) |
Taxonomic Lineage | cellular organisms Bacteria Terrabacteria group Actinobacteria Actinomycetia (high G+C Gram-positive bacteria) Micrococcales Cellulomonadaceae Cellulomonas Cellulomonas fimi Cellulomonas fimi (strain ATCC 484 / DSM 20113 / JCM 1341 / NBRC 15513 / NCIMB 8980 / NCTC 7547) |
Enzyme Sequence | MSTLGKRAGVRRRVRAVATAATATALVAVPLTTLATSASAAPVHVDNPYAGAVQYVNPTWAASVNAAAGRQSADPALAAKMRTVAGQPTAVWMDRISAITGNADGNGLKFHLDNAVAQQKAAGVPLVFNLVIYDLPGRDCFALASNGELPATDAGLARYKSEYIDPIADLLDNPEYESIRIAATIEPDSLPNLTTNISEPACQQAAPYYRQGVKYALDKLHAIPNVYNYIDIGHSGWLGWDSNAGPSATLFAEVAKSTTAGFASIDGFVSDVANTTPLEEPLLSDSSLTINNTPIRSSKFYEWNFDFDEIDYTAHMHRLLVAAGFPSSIGMLVDTSRNGWGGPNRPTSITASTDVNAYVDANRVDRRVHRGAWCNPLGAGIGRFPEATPSGYAASHLDAFVWIKPPGESDGASTDIPNDQGKRFDRMCDPTFVSPKLNNQLTGATPNAPLAGQWFEEQFVTLVKNAYPVIGGTTPVEDLVAPTVPTGLTAGTTTATSVPLSWTASTDNVAVTGYDVYRGTTLVGTTAATSYTVTGLTPATAYSFTVRAKDAAGNVSAASAAAAATTQSGTVTDTTAPSVPAGLTAGTTTTTTVPLSWTASTDNAGGSGVAGYEVLRGTTVVGTTTATSYTVTGLTAGTTYSFSVRAKDVAGNTSAASAAVSATTQTGTVVDTTAPSVPTGLTAGTTTTSSVPLTWTASTDNAGGSGVAGYEVFNGTTRVATVTSTSYTVTGLAADTAYSFTVKAKDVAGNVSAASAAVSARTQAATSGGCTVKYSASSWNTGFTGTVEVKNNGTAALNGWTLGFSFADGQKVSQGWSAEWSQSGTAVTAKNAPWNGTLAAGSSVSIGFNGTHNGTNTAPTAFTLNGVACTLG |
Enzyme Length | 872 |
Uniprot Accession Number | P50401 |
Absorption | |
Active Site | ACT_SITE 188; /note=Proton donor; /evidence=ECO:0000255|PROSITE-ProRule:PRU10057; ACT_SITE 410; /note=Nucleophile; /evidence=ECO:0000255|PROSITE-ProRule:PRU10056 |
Activity Regulation | |
Binding Site | |
Calcium Binding | |
catalytic Activity | CATALYTIC ACTIVITY: Reaction=Hydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose and cellotetraose, releasing cellobiose from the non-reducing ends of the chains.; EC=3.2.1.91; |
DNA Binding | |
EC Number | 3.2.1.91 |
Enzyme Function | FUNCTION: This enzyme hydrolyzes 1,4-beta-D-glucosidic linkages of cellulose. Weak activity against carboxymethylcellulose, bacterial microcrystalline cellulose and barley beta-glucan. Has also weak endoglucanase activity. Hydrolyzes glucosidic bonds with inversion of anomeric configuration. |
Temperature Dependency | |
PH Dependency | |
Pathway | |
nucleotide Binding | |
Features | Active site (2); Beta strand (12); Chain (1); Disulfide bond (3); Domain (4); Helix (19); Region (1); Signal peptide (1); Turn (4) |
Keywords | 3D-structure;Carbohydrate metabolism;Cellulose degradation;Direct protein sequencing;Disulfide bond;Glycosidase;Hydrolase;Polysaccharide degradation;Reference proteome;Repeat;Signal |
Interact With | |
Induction | |
Subcellular Location | |
Modified Residue | |
Post Translational Modification | |
Signal Peptide | SIGNAL 1..40; /evidence=ECO:0000269|PubMed:8458833 |
Structure 3D | X-ray crystallography (1) |
Cross Reference PDB | 7CBD; |
Mapped Pubmed ID | 32816991; |
Motif | |
Gene Encoded By | |
Mass | 89,301 |
Kinetics | |
Metal Binding | |
Rhea ID | |
Cross Reference Brenda |