| IED ID | IndEnz0011000053 |
| Enzyme Type ID | glucanase000053 |
| Protein Name |
Probable endo-1,3 4 -beta-glucanase AO090023000083 EC 3.2.1.6 Mixed-linked glucanase AO090023000083 |
| Gene Name | AO090023000083 |
| Organism | Aspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta Eurotiomycetes Eurotiomycetidae Eurotiales (green and blue molds) Aspergillaceae Aspergillus Aspergillus subgen. Circumdati Aspergillus oryzae (Yellow koji mold) Aspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) |
| Enzyme Sequence | MSSSSFVWTVGSIALSSLITPTIADGSGSRYQLTEAWQGEKFLDHFKFFSGSDPTNGFVTYANQSYAESSGLIEVTESGSFYMGVDYKTKLSPNGPGRDSVRIESKEYYDEGLYIIDLQHMPGSVCGTWPAFWSVGPNWPYDGEIDIIEGVNKHEANEIVLHTSGSCSLSSENDMSGTMTSSECGESSGTIGCVVKGQDGTSGAPFNEKNGGVYAMEWTSSFVKIWYFARSEIPQSITEGNPDTTAFGTPMAHLQGTCDFGERFKSQKFILDTTFCGDWAGGVFGDSGCPVSDPSNPIQSCVNYVAENPAAFKEAYWEINYIKLFQTGTGHSTASVASQAETATAVASNTVDSIPSVTSTAIPETTAPAPETVSAEAPATSSAVPEPANPQTSVAGAETTAAPAPSPETTAAPASPSLDDSDGADAVSETTIYVTETTTICGASTQKGTIQTIGGGETEVSPASSTVESAATPAAPTPTSQKPVASLPGTTVNGGTPVPTDVSPETPAEETAGESGAPTPSAEQPEQPQPAATSIETGTVPPAVSNPAPTEQGTPEGASPVDATESRHDSDEPAPTSAAPIRSPSPSSWTISSSSRVASSSSFASTTSSASRTTSATKEATAPTETDSGASTGTNPESPVFTAGASKSVGISGLTGIVCGIAMAMLA |
| Enzyme Length | 667 |
| Uniprot Accession Number | Q2UIE6 |
| Absorption | |
| Active Site | ACT_SITE 144; /note=Nucleophile; /evidence=ECO:0000250; ACT_SITE 149; /note=Proton donor; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Endohydrolysis of (1->3)- or (1->4)-linkages in beta-D-glucans when the glucose residue whose reducing group is involved in the linkage to be hydrolyzed is itself substituted at C-3.; EC=3.2.1.6; |
| DNA Binding | |
| EC Number | 3.2.1.6 |
| Enzyme Function | FUNCTION: Mixed-linked glucanase involved in the degradation of complex natural cellulosic substrates. {ECO:0000250}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Chain (1); Compositional bias (4); Domain (1); Glycosylation (1); Lipidation (1); Propeptide (1); Region (2); Signal peptide (1) |
| Keywords | Carbohydrate metabolism;Cell membrane;Cellulose degradation;GPI-anchor;Glycoprotein;Glycosidase;Hydrolase;Lipoprotein;Membrane;Polysaccharide degradation;Reference proteome;Signal |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Lipid-anchor, GPI-anchor {ECO:0000250}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..24; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 68,301 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |