IED ID | IndEnz0011000058 |
Enzyme Type ID | glucanase000058 |
Protein Name |
Endoglucanase 1 EC 3.2.1.4 Cellulase 1 Endo-1,4-beta-glucanase 1 |
Gene Name | egl1 MGG_02532 |
Organism | Magnaporthe oryzae (strain 70-15 / ATCC MYA-4617 / FGSC 8958) (Rice blast fungus) (Pyricularia oryzae) |
Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta sordariomyceta Sordariomycetes Sordariomycetidae Magnaporthales Pyriculariaceae Pyricularia Magnaporthe oryzae (Rice blast fungus) (Pyricularia oryzae) Magnaporthe oryzae (strain 70-15 / ATCC MYA-4617 / FGSC 8958) (Rice blast fungus) (Pyricularia oryzae) |
Enzyme Sequence | MAKFSALCSLALLGLATAQKPVGTETHEKLTTFRCTVAGGCVEKTNYIVLDSITGHRIYQPAAETLDCGARGAAPNVTACPTKEACAENCAMEGRTDYGSQGVSTDGASLRLQILHDGKKVAPRVYLLDETEAKYEMLRLTGNEFAFEVTMDKLPCGMNSALYLSEMEEDGGKSELNPGGAPWGTGYCDAQCYVTPFINGEGNIKGNGACCAEMDIWEANSRATHIAPHPCSKPGLYLCEGDECGSTGVCDKSGCAWNPNRIAQPHYYGNNDTFKVDTLKPMTVVTQFPTDASGKLAAIRRLYVQGGVVIKAETVHKAGLPEVDALTDPFCEAFGSQRYMALGATGGMGDALARGMVLVMSIWWDETGGNMQWLDGIASGSGPCNATEGAPANIPLVEPNPEVTFSNLKWGEIGSTFQGGARRL |
Enzyme Length | 424 |
Uniprot Accession Number | G4NK46 |
Absorption | |
Active Site | ACT_SITE 213; /note=Nucleophile; /evidence=ECO:0000250|UniProtKB:P56680; ACT_SITE 218; /note=Proton donor; /evidence=ECO:0000250|UniProtKB:P56680 |
Activity Regulation | |
Binding Site | |
Calcium Binding | |
catalytic Activity | CATALYTIC ACTIVITY: Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4; Evidence={ECO:0000269|PubMed:18247030}; |
DNA Binding | |
EC Number | 3.2.1.4 |
Enzyme Function | FUNCTION: Endoglucanase that is involved in the biological conversion of cellulose to glucose. Hydrolyses internal beta-1,4-glucosidic bonds. {ECO:0000269|PubMed:18247030}. |
Temperature Dependency | |
PH Dependency | |
Pathway | |
nucleotide Binding | |
Features | Active site (2); Chain (1); Disulfide bond (9); Glycosylation (3); Signal peptide (1) |
Keywords | Carbohydrate metabolism;Cellulose degradation;Disulfide bond;Glycoprotein;Glycosidase;Hydrolase;Polysaccharide degradation;Reference proteome;Secreted;Signal |
Interact With | |
Induction | INDUCTION: Expression is increased at 72 h of infection, and then the expression level drops back to the lower level for the remainder of the time course. {ECO:0000269|PubMed:18247030}. |
Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:18247030}. |
Modified Residue | |
Post Translational Modification | |
Signal Peptide | SIGNAL 1..18; /evidence=ECO:0000255 |
Structure 3D | |
Cross Reference PDB | - |
Mapped Pubmed ID | - |
Motif | |
Gene Encoded By | |
Mass | 45,062 |
Kinetics | |
Metal Binding | |
Rhea ID | |
Cross Reference Brenda |