IED ID | IndEnz0011000059 |
Enzyme Type ID | glucanase000059 |
Protein Name |
Endo-beta-1,4-glucanase Endoglucanase EC 3.2.1.4 Carboxymethylcellulase Cellulase Fragment |
Gene Name | |
Organism | Aspergillus versicolor |
Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta Eurotiomycetes Eurotiomycetidae Eurotiales (green and blue molds) Aspergillaceae Aspergillus Aspergillus subgen. Nidulantes Aspergillus versicolor |
Enzyme Sequence | VAAIQTVLG |
Enzyme Length | 9 |
Uniprot Accession Number | C0HJH0 |
Absorption | |
Active Site | |
Activity Regulation | |
Binding Site | |
Calcium Binding | |
catalytic Activity | CATALYTIC ACTIVITY: Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4; Evidence={ECO:0000269|Ref.1}; |
DNA Binding | |
EC Number | 3.2.1.4 |
Enzyme Function | FUNCTION: Has endoglucanase activity on substrates containing beta-1,4 glycosidic bonds, like in carboxymethylcellulose (CMC). {ECO:0000269|Ref.1}. |
Temperature Dependency | BIOPHYSICOCHEMICAL PROPERTIES: Temperature dependence: Optimum temperature is 30 degrees Celsius. {ECO:0000269|Ref.1}; |
PH Dependency | BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 4.0. {ECO:0000269|Ref.1}; |
Pathway | |
nucleotide Binding | |
Features | Chain (1); Non-terminal residue (1) |
Keywords | Carbohydrate metabolism;Cellulose degradation;Direct protein sequencing;Glycosidase;Hydrolase;Polysaccharide degradation;Secreted |
Interact With | |
Induction | INDUCTION: Induced by Co(2+), Ba(2+), Mn(2+), Mg(2+) and Cu(2+) ions. Repressed by Hg(2+) ions and methanol. {ECO:0000269|Ref.1}. |
Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000269|Ref.1}. |
Modified Residue | |
Post Translational Modification | |
Signal Peptide | |
Structure 3D | |
Cross Reference PDB | - |
Mapped Pubmed ID | - |
Motif | |
Gene Encoded By | |
Mass | 871 |
Kinetics | BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=1.134 mM for carboxymethylcellulose {ECO:0000269|Ref.1}; |
Metal Binding | |
Rhea ID | |
Cross Reference Brenda |