Detail Information for IndEnz0011000061
IED ID IndEnz0011000061
Enzyme Type ID glucanase000061
Protein Name Probable endo-beta-1,4-glucanase D
Endoglucanase D
EC 3.2.1.4
Carboxymethylcellulase D
Cellulase D
Gene Name eglD An15g04900
Organism Aspergillus niger (strain CBS 513.88 / FGSC A1513)
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta Eurotiomycetes Eurotiomycetidae Eurotiales (green and blue molds) Aspergillaceae Aspergillus Aspergillus subgen. Circumdati Aspergillus niger Aspergillus niger (strain CBS 513.88 / FGSC A1513)
Enzyme Sequence MKTTTYSLLALAAASKLASAHTTVQAVWINGEDQGLGNSADGYIRSPPSNSPVTDVTSTDMTCNVNGDQAASKTLSVKAGDVVTFEWHHSDRSDSDDIIASSHKGPVQVYMAPTAKGSNGNNWVKIAEDGYHKSSDEWATDILIANKGKHNITVPDVPAGNYLFRPEIIALHEGNREGGAQFYMECVQFKVTSDGSSELPSGVSIPGVYTATDPGILFDIYNSFDSYPIPGPDVWDGSSSGSSSGSSSAAAAATTSAAVAATTPATQAAVEVSSSAAAVVESTSSAAAATTEAAAPVVSSAAPVQQATSAVTSQAQAPTTFATSSKSSKTACKNKTKSKSKVAASSTEAVVAPAPTSSVVPAVSASASASAGGVAKMYERCGGINHTGPTTCESGSVCKKWNPYYYQCVASQ
Enzyme Length 412
Uniprot Accession Number A2R5N0
Absorption
Active Site ACT_SITE 167; /note=Proton donor; /evidence=ECO:0000250; ACT_SITE 213; /note=Nucleophile; /evidence=ECO:0000250
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4;
DNA Binding
EC Number 3.2.1.4
Enzyme Function FUNCTION: Has endoglucanase activity on substrates containing beta-1,4 glycosidic bonds, like in carboxymethylcellulose (CMC), hydroxyethylcellulose (HEC) and beta-glucan. Involved in the degradation of complex natural cellulosic substrates (By similarity). {ECO:0000250}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (2); Chain (1); Disulfide bond (2); Domain (1); Glycosylation (3); Region (2); Signal peptide (1)
Keywords Carbohydrate metabolism;Cellulose degradation;Disulfide bond;Glycoprotein;Glycosidase;Hydrolase;Polysaccharide degradation;Reference proteome;Secreted;Signal
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
Modified Residue
Post Translational Modification
Signal Peptide SIGNAL 1..20; /evidence=ECO:0000255
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 41,981
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda