| IED ID | IndEnz0011000062 |
| Enzyme Type ID | glucanase000062 |
| Protein Name |
Aspartic protease EC 3.4.23.- |
| Gene Name | pr1 |
| Organism | Phaffia rhodozyma (Yeast) (Xanthophyllomyces dendrorhous) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Basidiomycota Agaricomycotina Tremellomycetes Cystofilobasidiales Mrakiaceae Phaffia Phaffia rhodozyma (Yeast) (Xanthophyllomyces dendrorhous) |
| Enzyme Sequence | MISDTVIAILAVALVGSTVQAAPVDATATSTSGIIAVPISKSAAQLAREADPVVSLDWLKKTKAQAQYKHKQANARLHSKRATGASVLTDQGSESLWTGPITIGGQSFTVDWDTGSSDLWVPSSACSSAACNAHHKYTLTSTGKKQSGTFSISYGDGSSASGPVYKDNVVASGLQATSQVFGAVTSESSSFSSDPSDGISGLGWPALAQLSGTSYFWSLINQGTVTSPVFSFRLATTNSELYLGGINSAHYTGAITYTPVTQKAYWTIALGGVSVNGAAINPSVSSAIIDTGTTLVYGPTAGVAALYAKIPGSASMADTYGSDYQGYYTFPCSAVPTVALTFGGSSFSVPTSAFNLGTVSSGSKQCVGGIVGQGDGSWLVGDVFLQGVYSIYDVGNARVGFAKTV |
| Enzyme Length | 405 |
| Uniprot Accession Number | O60020 |
| Absorption | |
| Active Site | ACT_SITE 113; /evidence="ECO:0000250|UniProtKB:P00790, ECO:0000255|PROSITE-ProRule:PRU10094"; ACT_SITE 290; /evidence="ECO:0000250|UniProtKB:P00790, ECO:0000255|PROSITE-ProRule:PRU10094" |
| Activity Regulation | ACTIVITY REGULATION: Inhibited by pepstatin A. {ECO:0000269|PubMed:10091328}. |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.23.- |
| Enzyme Function | FUNCTION: Possesses acidic protease activity. Hydrolyzes casein and azoalbumin in vitro. {ECO:0000269|PubMed:10091328}. |
| Temperature Dependency | BIOPHYSICOCHEMICAL PROPERTIES: Temperature dependence: Optimum temperature is approximately 40 degrees Celsius. {ECO:0000269|PubMed:10091328}; |
| PH Dependency | BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 4-6. {ECO:0000269|PubMed:10091328}; |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Chain (1); Disulfide bond (2); Domain (1); Propeptide (1); Signal peptide (1) |
| Keywords | Aspartyl protease;Cleavage on pair of basic residues;Direct protein sequencing;Disulfide bond;Hydrolase;Protease;Secreted;Signal;Zymogen |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:10091328}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..21; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 41,370 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |