IED ID | IndEnz0011000062 |
Enzyme Type ID | glucanase000062 |
Protein Name |
Aspartic protease EC 3.4.23.- |
Gene Name | pr1 |
Organism | Phaffia rhodozyma (Yeast) (Xanthophyllomyces dendrorhous) |
Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Basidiomycota Agaricomycotina Tremellomycetes Cystofilobasidiales Mrakiaceae Phaffia Phaffia rhodozyma (Yeast) (Xanthophyllomyces dendrorhous) |
Enzyme Sequence | MISDTVIAILAVALVGSTVQAAPVDATATSTSGIIAVPISKSAAQLAREADPVVSLDWLKKTKAQAQYKHKQANARLHSKRATGASVLTDQGSESLWTGPITIGGQSFTVDWDTGSSDLWVPSSACSSAACNAHHKYTLTSTGKKQSGTFSISYGDGSSASGPVYKDNVVASGLQATSQVFGAVTSESSSFSSDPSDGISGLGWPALAQLSGTSYFWSLINQGTVTSPVFSFRLATTNSELYLGGINSAHYTGAITYTPVTQKAYWTIALGGVSVNGAAINPSVSSAIIDTGTTLVYGPTAGVAALYAKIPGSASMADTYGSDYQGYYTFPCSAVPTVALTFGGSSFSVPTSAFNLGTVSSGSKQCVGGIVGQGDGSWLVGDVFLQGVYSIYDVGNARVGFAKTV |
Enzyme Length | 405 |
Uniprot Accession Number | O60020 |
Absorption | |
Active Site | ACT_SITE 113; /evidence="ECO:0000250|UniProtKB:P00790, ECO:0000255|PROSITE-ProRule:PRU10094"; ACT_SITE 290; /evidence="ECO:0000250|UniProtKB:P00790, ECO:0000255|PROSITE-ProRule:PRU10094" |
Activity Regulation | ACTIVITY REGULATION: Inhibited by pepstatin A. {ECO:0000269|PubMed:10091328}. |
Binding Site | |
Calcium Binding | |
catalytic Activity | |
DNA Binding | |
EC Number | 3.4.23.- |
Enzyme Function | FUNCTION: Possesses acidic protease activity. Hydrolyzes casein and azoalbumin in vitro. {ECO:0000269|PubMed:10091328}. |
Temperature Dependency | BIOPHYSICOCHEMICAL PROPERTIES: Temperature dependence: Optimum temperature is approximately 40 degrees Celsius. {ECO:0000269|PubMed:10091328}; |
PH Dependency | BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 4-6. {ECO:0000269|PubMed:10091328}; |
Pathway | |
nucleotide Binding | |
Features | Active site (2); Chain (1); Disulfide bond (2); Domain (1); Propeptide (1); Signal peptide (1) |
Keywords | Aspartyl protease;Cleavage on pair of basic residues;Direct protein sequencing;Disulfide bond;Hydrolase;Protease;Secreted;Signal;Zymogen |
Interact With | |
Induction | |
Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:10091328}. |
Modified Residue | |
Post Translational Modification | |
Signal Peptide | SIGNAL 1..21; /evidence=ECO:0000255 |
Structure 3D | |
Cross Reference PDB | - |
Mapped Pubmed ID | - |
Motif | |
Gene Encoded By | |
Mass | 41,370 |
Kinetics | |
Metal Binding | |
Rhea ID | |
Cross Reference Brenda |