Detail Information for IndEnz0011000069
IED ID IndEnz0011000069
Enzyme Type ID glucanase000069
Protein Name Glucan endo-1,3-beta-glucosidase 5
EC 3.2.1.39
1-
3
-beta-glucan endohydrolase 5
1-
3
-beta-glucanase 5
Beta-1,3-endoglucanase 5
Beta-1,3-glucanase 5
Gene Name At4g31140 F6E21.60
Organism Arabidopsis thaliana (Mouse-ear cress)
Taxonomic Lineage cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress)
Enzyme Sequence MLFKGVFAVFFVITLLYASLLIEVEGIGVNWGSQARHPLPPATVVRLLRENGIQKVKLFEADSAILKALSRTGIQVMVGIPNDLLAPLAGSVAAAERWVSQNVSAHVSSNGVDIRYVAVGNEPFLKAFNGTFEGITLPALQNIQSAIIKAGLATQVKVTVPLNADVYQSASNLPSDGDFRPEIRDLMLNIVKFLSDNGAPFTINIYPFISLYNDPNFPVEFAFFDGTGTPINDNGRIYDNVLDANYDTLVWSLQKNGFGNLTIIVGEVGWPTDGDKNANLMYARRYNQGFMNRQKANKGTPMRPGAMDAYLFGLIDEDAKSIQPGNFERHWGIFYIDGQPKYQLSLGSGNGLIPAKDVHYLAKKWCILAPNANLQDPQLGPSVSYACDHADCTSLGYGSSCGNLNLAQNVSYAFNSYYQVSNQLDSACKFPGLSIVSTRDPSVGSCKFKIMIKSEDASEASAMMPITRSTAVLLLLSICLYIVL
Enzyme Length 484
Uniprot Accession Number Q9M088
Absorption
Active Site ACT_SITE 122; /note=Proton donor; /evidence=ECO:0000250|UniProtKB:O22317; ACT_SITE 267; /note=Nucleophile; /evidence=ECO:0000250|UniProtKB:O22317
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans.; EC=3.2.1.39;
DNA Binding
EC Number 3.2.1.39
Enzyme Function
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (2); Chain (1); Disulfide bond (1); Glycosylation (4); Lipidation (1); Propeptide (1); Signal peptide (1)
Keywords Cell membrane;Disulfide bond;GPI-anchor;Glycoprotein;Glycosidase;Hydrolase;Lipoprotein;Membrane;Plant defense;Reference proteome;Signal
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Cell membrane; Lipid-anchor, GPI-anchor.
Modified Residue
Post Translational Modification PTM: Contains two additional disulfide bonds. {ECO:0000250}.
Signal Peptide SIGNAL 1..26; /evidence=ECO:0000255
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID 12068095; 12805588; 15215502; 15235117; 16618929; 17317660; 17363895; 17526915; 17644812; 18684332;
Motif
Gene Encoded By
Mass 52,715
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda