Detail Information for IndEnz0011000071
IED ID IndEnz0011000071
Enzyme Type ID glucanase000071
Protein Name Glucan endo-1,3-beta-glucosidase 7
EC 3.2.1.39
1-
3
-beta-glucan endohydrolase 7
1-
3
-beta-glucanase 7
Beta-1,3-endoglucanase 7
Beta-1,3-glucanase 7
Gene Name At4g34480 T4L20.60
Organism Arabidopsis thaliana (Mouse-ear cress)
Taxonomic Lineage cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress)
Enzyme Sequence MALSISIYFLLIFLSHFPSSHAEPFIGVNYGQVADNLPPPSETVKLLQSTSIQKVRLYGADPAIIKALAGTGVGIVIGAANGDVPSLASDPNAATQWINSNVLPFYPASKIMLITVGNEILMSNDPNLVNQLLPAMQNVQKALEAVSLGGKIKVSTVNSMTVLGSSDPPSSGSFAAGYQTGLKGILQFLSDTGSPFAINPYPFFAYQSDPRPETLAFCLFEPNAGRVDSKTGIKYTNMFDAQVDAVHSALKSMGFEKVEIVVAETGWASRGDANEVGASVDNAKAYNGNLIAHLRSMVGTPLMPGKPVDTYIFALYDENLKPGPSSERAFGLFKTDLSMVYDVGLAKSSSSSQTPSGKVTSSGWCVPKKGATNEELQASLDWACGHGIDCGAIQPGGACFEPNNVVSHAAYAMNMYFQKSPKQPTDCDFSKTATVTSQNPSYNNCVYPGGGGGGGGGGGGSKAVMNKYVSSDKVEKKNGATEPKVSSSLSFLLIFLSLIFHVYM
Enzyme Length 504
Uniprot Accession Number Q9M069
Absorption
Active Site ACT_SITE 119; /note=Proton donor; /evidence=ECO:0000250|UniProtKB:O22317; ACT_SITE 264; /note=Nucleophile; /evidence=ECO:0000250|UniProtKB:O22317
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans.; EC=3.2.1.39;
DNA Binding
EC Number 3.2.1.39
Enzyme Function
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (2); Chain (1); Disulfide bond (1); Erroneous gene model prediction (2); Signal peptide (1)
Keywords Cell wall;Cell wall biogenesis/degradation;Direct protein sequencing;Disulfide bond;Glycosidase;Hydrolase;Reference proteome;Secreted;Signal
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:9188482}. Secreted, cell wall {ECO:0000269|PubMed:9188482}.
Modified Residue
Post Translational Modification PTM: Contains two additional disulfide bonds. {ECO:0000250}.
Signal Peptide SIGNAL 1..22; /evidence=ECO:0000269|PubMed:9188482
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID 15047898; 16709196; 17470057; 18650403; 23738689; 28627464;
Motif
Gene Encoded By
Mass 53,121
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda