Detail Information for IndEnz0011000072
IED ID IndEnz0011000072
Enzyme Type ID glucanase000072
Protein Name Glucan endo-1,3-beta-glucosidase 8
EC 3.2.1.39
1-
3
-beta-glucan endohydrolase 8
1-
3
-beta-glucanase 8
Beta-1,3-endoglucanase 8
Beta-1,3-glucanase 8
Gene Name At1g64760 F13O11.7
Organism Arabidopsis thaliana (Mouse-ear cress)
Taxonomic Lineage cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress)
Enzyme Sequence MSNLLALVVGFVIVIGHLGILVNGLGVNWGTMATHKLPPKTVVQMLKDNNINKVKLFDADETTMGALAGSGLEVMVAIPNDQLKVMTSYDRAKDWVRKNVTRYNFDGGVNITFVAVGNEPFLKSYNGSFINLTFPALANIQNALNEAGLGNSVKATVPLNADVYDSPASNPVPSAGRFRPDIIGQMTQIVDFLGKNNAPITINIYPFLSLYGNDDFPLNYAFFDGAEPINDNGIDYTNVFDANFDTLVSSLKAVGHGDMPIIVGEVGWPTEGDKHANAGSAYRFYNGLLPRLGTNKGTPLRPTYIEVYLFGLLDEDAKSIAPGPFERHWGIFKFDGQPKFPIDLSGQGQSKFLIGAQNVPYLPNKWCTFNPEAKDLTKLAANIDYACTFSDCTALGYGSSCNTLDANGNASYAFNMFFQVKNQDESACYFQGLATITTQNISQGQCNFPIQIVASSASSFSCSSYSLVVLIVWFLLSGMMF
Enzyme Length 481
Uniprot Accession Number Q6NKW9
Absorption
Active Site ACT_SITE 119; /note=Proton donor; /evidence=ECO:0000250|UniProtKB:O22317; ACT_SITE 265; /note=Nucleophile; /evidence=ECO:0000250|UniProtKB:O22317
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans.; EC=3.2.1.39;
DNA Binding
EC Number 3.2.1.39
Enzyme Function
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (2); Chain (1); Disulfide bond (1); Erroneous initiation (1); Glycosylation (6); Lipidation (1); Propeptide (1); Sequence conflict (4); Signal peptide (1)
Keywords Cell membrane;Cell wall;Cell wall biogenesis/degradation;Disulfide bond;GPI-anchor;Glycoprotein;Glycosidase;Hydrolase;Lipoprotein;Membrane;Plant defense;Reference proteome;Secreted;Signal
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Secreted, cell wall {ECO:0000305}. Cell membrane; Lipid-anchor, GPI-anchor; Extracellular side.
Modified Residue
Post Translational Modification PTM: Contains two additional disulfide bonds. {ECO:0000250}.
Signal Peptide SIGNAL 1..33; /evidence=ECO:0000255
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID 12068095; 12805588; 14517339; 14671020; 17317660; 17644812; 20736450; 21917552; 28507000; 31113822;
Motif
Gene Encoded By
Mass 52,281
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda