IED ID | IndEnz0011000075 |
Enzyme Type ID | glucanase000075 |
Protein Name |
Glucan endo-1,3-beta-glucosidase GI EC 3.2.1.39 1- 3 -beta-glucan endohydrolase GI 1- 3 -beta-glucanase isoenzyme GI Beta-1,3-endoglucanase GI |
Gene Name | |
Organism | Hordeum vulgare (Barley) |
Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae Liliopsida Petrosaviidae commelinids Poales Poaceae BOP clade Pooideae Triticodae Triticeae Hordeinae Hordeum Hordeum vulgare (Barley) |
Enzyme Sequence | TIGVCYGVVANNLPPANEVVQLYRSNGLTGMRIYFADAKALSALRGSGIGLILDVGGNDVLASLAANASNAANWVRDNVRPYYPAVNIKYIAAGNEVWGGDTQNIVPAMRNLGAALKAPGLGTIKVSTSIRFDAVTNTFPPSNGVFAQAYMTDVARLLASTGAPLLTNVYPYFAYKDNPRDIQLNYATFRPGTTTVRDPNTGLTSQCLFDAMVDAVVAALERSGAPGVRVVVSESGWPSASGFAATADNARAYNQGLIDHVGGGTPKRPGALETYIFAMFNENFKTGELTEKHFGLFNPDKSPAYPIRFQ |
Enzyme Length | 310 |
Uniprot Accession Number | P34742 |
Absorption | |
Active Site | ACT_SITE 96; /note=Proton donor; /evidence=ECO:0000250|UniProtKB:O22317; ACT_SITE 234; /note=Nucleophile; /evidence=ECO:0000250|UniProtKB:O22317 |
Activity Regulation | |
Binding Site | |
Calcium Binding | |
catalytic Activity | CATALYTIC ACTIVITY: Reaction=Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans.; EC=3.2.1.39; |
DNA Binding | |
EC Number | 3.2.1.39 |
Enzyme Function | FUNCTION: May provide a degree of protection against microbial invasion of germinated barley grain through its ability to degrade fungal cell wall polysaccharides. Does not hydrolyze (1,3;1,4)-beta-D-glucans, (1,6)-beta-D-glucan, CM-cellulose, insoluble (1,3)-beta-D-glucans or aryl beta-D-glycosides. |
Temperature Dependency | |
PH Dependency | BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 4.8.; |
Pathway | |
nucleotide Binding | |
Features | Active site (2); Chain (1); Sequence conflict (2) |
Keywords | Direct protein sequencing;Glycosidase;Hydrolase;Plant defense |
Interact With | |
Induction | |
Subcellular Location | |
Modified Residue | |
Post Translational Modification | |
Signal Peptide | |
Structure 3D | |
Cross Reference PDB | - |
Mapped Pubmed ID | - |
Motif | |
Gene Encoded By | |
Mass | 32,954 |
Kinetics | |
Metal Binding | |
Rhea ID | |
Cross Reference Brenda |