| IED ID | IndEnz0011000082 |
| Enzyme Type ID | glucanase000082 |
| Protein Name |
Glucan endo-1,3-beta-glucosidase EC 3.2.1.39 1- 3 -beta-glucan endohydrolase 1- 3 -beta-glucanase Beta-1,3-endoglucanase |
| Gene Name | BGL |
| Organism | Brassica campestris (Field mustard) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Brassiceae Brassica Brassica campestris (Field mustard) |
| Enzyme Sequence | MLASSPMLLFLLSLLMAYNFDTTAGQIGVCFGQMGNNIPNPSEVVAMFKQYSIPRMRMYGPNPDALNALRGSNIEFILDVPNGDLKRLADSQAEANTWVRDNVQKYNDVRFKYISVGNEVKPGEPGAAALIQAMQNIDRALSAAGLSNIKVSTTTFMGPSRNTYPPSRGRFKDEYRNFLQPVIGFLVNKRSPLLVNIYTYFGYMNRDVSLQFALLQPNSNNEFTDPNNQLRYLNFFDANLDSVYAALEKSGGGSLDVVVSESGWPTQGGPGASVPNAEAYVNNLRLHVNKNGSPKRQEAIETYIFAMFDEAPRQTSPNDEYEKYWGMFSPTTRQLKYGVKFN |
| Enzyme Length | 342 |
| Uniprot Accession Number | P49236 |
| Absorption | |
| Active Site | ACT_SITE 119; /note=Proton donor; /evidence=ECO:0000250|UniProtKB:O22317; ACT_SITE 261; /note=Nucleophile; /evidence=ECO:0000250|UniProtKB:O22317 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans.; EC=3.2.1.39; |
| DNA Binding | |
| EC Number | 3.2.1.39 |
| Enzyme Function | FUNCTION: Is thought to be an important plant defense-related product against fungal pathogens. Accumulation of the glucanase can be detected as early as 4 hours after inoculation. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Chain (1); Signal peptide (1) |
| Keywords | Direct protein sequencing;Glycosidase;Hydrolase;Plant defense;Signal;Vacuole |
| Interact With | |
| Induction | INDUCTION: Following incompatible or compatible interaction with pathogenic bacteria, but not by wounding or salicylic acid. The induction is localized and does not seem to result from a systemic response. Induction occurs more rapidly with an incompatible interaction. |
| Subcellular Location | SUBCELLULAR LOCATION: Vacuole. Note=In intact tissues. |
| Modified Residue | |
| Post Translational Modification | PTM: The N-terminus is blocked. |
| Signal Peptide | SIGNAL 1..26; /evidence=ECO:0000250 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 38,180 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |