| IED ID | IndEnz0011000092 |
| Enzyme Type ID | glucanase000092 |
| Protein Name |
Probable glucan 1,3-beta-glucosidase ARB_04467 EC 2.4.1.- EC 3.2.1.58 Exo-1,3-beta-glucanase |
| Gene Name | ARB_04467 |
| Organism | Arthroderma benhamiae (strain ATCC MYA-4681 / CBS 112371) (Trichophyton mentagrophytes) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta Eurotiomycetes Eurotiomycetidae Onygenales Arthrodermataceae (dermatophytes) Trichophyton Arthroderma benhamiae (Trichophyton mentagrophytes) Arthroderma benhamiae (strain ATCC MYA-4681 / CBS 112371) (Trichophyton mentagrophytes) |
| Enzyme Sequence | MKFGSLLGLSLVGLSVASPVTNVWKSPRAADDFIRGVNLGGWLVLEPWITPGIFEEGGDSAVDEWTLSAALGHRAHERLKLHWNTFMEQKDFDRIKGAGLTHVRIPIGYWAVAPIQGEPFVQGQVDMLDAAIDWARHSGLKVNVDLHGAPGSQNGFDNSGRLGPANWQKGDTVAQTYKALDVLIQRYAKKDGVVDEINLINEPFPQAGIQVEPLKDYYRQGAAKVKSANPNVAVVISDAFMGPSKWNGFDVGAKTIIDTHHYQVFSPQLVAMDINQHVKAACDFGNDELAKSSIPAIVGEWCGALTDCTQYLNGRHEGARYDGTHKDSDPKTAVPNGCVRKTGGSASQLTDEEKTNTRRYIEAQLDSFSKGHGWFWWTWKTERGSPGWDLNDLLSNGLFPQPLDSRMFLGQCN |
| Enzyme Length | 413 |
| Uniprot Accession Number | D4AJL7 |
| Absorption | |
| Active Site | ACT_SITE 202; /note=Proton donor; /evidence=ECO:0000250|UniProtKB:O85465; ACT_SITE 300; /note=Nucleophile; /evidence=ECO:0000250|UniProtKB:P29717 |
| Activity Regulation | |
| Binding Site | BINDING 46; /note=Substrate; /evidence=ECO:0000250|UniProtKB:P29717; BINDING 202; /note=Substrate; /evidence=ECO:0000250|UniProtKB:P29717; BINDING 262; /note=Substrate; /evidence=ECO:0000250|UniProtKB:P29717 |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Successive hydrolysis of beta-D-glucose units from the non-reducing ends of (1->3)-beta-D-glucans, releasing alpha-glucose.; EC=3.2.1.58; Evidence={ECO:0000250|UniProtKB:P29717}; |
| DNA Binding | |
| EC Number | 2.4.1.-; 3.2.1.58 |
| Enzyme Function | FUNCTION: Major glucan 1,3-beta-glucosidase required for cell wall integrity (By similarity). Beta-glucanases participate in the metabolism of beta-glucan, the main structural component of the cell wall (By similarity). Can also function biosynthetically as a transglycosylase (By similarity). Functions to deliver glucan from the cell to the extracellular matrix (By similarity). Involved in cell-substrate and cell-cell adhesion (By similarity). {ECO:0000250|UniProtKB:P29717}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Binding site (3); Chain (1); Disulfide bond (1); Signal peptide (1) |
| Keywords | Cell adhesion;Cell wall;Cell wall biogenesis/degradation;Disulfide bond;Glycosidase;Hydrolase;Reference proteome;Secreted;Signal;Transferase |
| Interact With | |
| Induction | INDUCTION: Expression is down-regulated in presence of human keratinocytes. {ECO:0000269|PubMed:21247460}. |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:21247460, ECO:0000269|PubMed:21919205}. Secreted, cell wall {ECO:0000250|UniProtKB:P29717}. Note=Is non-covalently attached to the cell wall. {ECO:0000250|UniProtKB:P29717}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..17; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 45,416 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |