Detail Information for IndEnz0011000098
IED ID IndEnz0011000098
Enzyme Type ID glucanase000098
Protein Name Endoglucanase E1
EC 3.2.1.4
Cellulase E1
Endo-1,4-beta-glucanase E1
Endocellulase E1
Gene Name Acel_0614
Organism Acidothermus cellulolyticus (strain ATCC 43068 / DSM 8971 / 11B)
Taxonomic Lineage cellular organisms Bacteria Terrabacteria group Actinobacteria Actinomycetia (high G+C Gram-positive bacteria) Acidothermales Acidothermaceae Acidothermus Acidothermus cellulolyticus Acidothermus cellulolyticus (strain ATCC 43068 / DSM 8971 / 11B)
Enzyme Sequence MPRALRRVPGSRVMLRVGVVVAVLALVAALANLAVPRPARAAGGGYWHTSGREILDANNVPVRIAGINWFGFETCNYVVHGLWSRDYRSMLDQIKSLGYNTIRLPYSDDILKPGTMPNSINFYQMNQDLQGLTSLQVMDKIVAYAGQIGLRIILDRHRPDCSGQSALWYTSSVSEATWISDLQALAQRYKGNPTVVGFDLHNEPHDPACWGCGDPSIDWRLAAERAGNAVLSVNPNLLIFVEGVQSYNGDSYWWGGNLQGAGQYPVVLNVPNRLVYSAHDYATSVYPQTWFSDPTFPNNMPGIWNKNWGYLFNQNIAPVWLGEFGTTLQSTTDQTWLKTLVQYLRPTAQYGADSFQWTFWSWNPDSGDTGGILKDDWQTVDTVKDGYLAPIKSSIFDPVGASASPSSQPSPSVSPSPSPSPSASRTPTPTPTPTASPTPTLTPTATPTPTASPTPSPTAASGARCTASYQVNSDWGNGFTVTVAVTNSGSVATKTWTVSWTFGGNQTITNSWNAAVTQNGQSVTARNMSYNNVIQPGQNTTFGFQASYTGSNAAPTVACAAS
Enzyme Length 562
Uniprot Accession Number P54583
Absorption
Active Site ACT_SITE 203; /note=Proton donor; ACT_SITE 323; /note=Nucleophile
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4;
DNA Binding
EC Number 3.2.1.4
Enzyme Function FUNCTION: Has a very high specific activity on carboxymethylcellulose.
Temperature Dependency BIOPHYSICOCHEMICAL PROPERTIES: Temperature dependence: Optimum temperature is 81 degrees Celsius. Thermostable.;
PH Dependency
Pathway
nucleotide Binding
Features Active site (2); Beta strand (17); Chain (1); Compositional bias (1); Disulfide bond (2); Domain (1); Helix (12); Region (2); Signal peptide (1); Turn (9)
Keywords 3D-structure;Carbohydrate metabolism;Cellulose degradation;Disulfide bond;Glycosidase;Hydrolase;Polysaccharide degradation;Reference proteome;Signal
Interact With
Induction
Subcellular Location
Modified Residue
Post Translational Modification
Signal Peptide SIGNAL 1..41
Structure 3D X-ray crystallography (2)
Cross Reference PDB 1ECE; 1VRX;
Mapped Pubmed ID 15917594;
Motif
Gene Encoded By
Mass 60,748
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda